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RIPK1 antibody (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1) (N-Term)

Details for Product anti-RIPK1 Antibody No. ABIN500614, Supplier: Login to see
Antigen
  • C9orf12
  • RGD1311271
  • rIpk1
  • RIP
  • RIP1
  • D330015H01Rik
  • Rinp
  • Rip1
  • 2400006N03Rik
  • RP23-83I13.5
  • Rip
Alternatives
anti-Human RIPK1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
N-Term
29
15
8
7
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
114
31
31
6
2
2
Host
Rabbit
85
25
4
Clonality
Polyclonal
Conjugate
This RIPK1 antibody is un-conjugated
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
92
38
36
22
21
12
6
2
1
1
Supplier
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Immunogen RIPK1 antibody was raised against a 15 amino acid peptide from near the amino terminus of human RIPK1.
Isotype IgG
Specificity This antibody detects RIP.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name RIP (RIPK1 Antibody Abstract)
Background RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.Synonyms: Cell death protein RIP, RIPK1, Receptor-Interacting Protein, Receptor-interacting serine/threonine-protein kinase 1, Serine/threonine-protein kinase RIP
Gene ID 8737
NCBI Accession NP_003795
UniProt Q13546
Research Area Cancer, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RIPK1 antibody (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1) (N-Term) (ABIN500614) Immunohistochemistry of RIPK1 in mouse kidney tissue with this product at 2.5 μg/ml.
Western Blotting (WB) image for anti-RIPK1 antibody (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1) (N-Term) (ABIN500614) Western blot analysis of RIPK1 in rat kidney tissue lysate with this product at 1 μg/ml.
Background publications Festjens, Vanden Berghe, Cornelis et al.: "RIP1, a kinase on the crossroads of a cell's decision to live or die." in: Cell death and differentiation, Vol. 14, Issue 3, pp. 400-10, 2007 (PubMed).

Meylan, Burns, Hofmann et al.: "RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation." in: Nature immunology, Vol. 5, Issue 5, pp. 503-7, 2004 (PubMed).

Hsu, Huang, Shu et al.: "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex." in: Immunity, Vol. 4, Issue 4, pp. 387-96, 1996 (PubMed).

Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).