Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (N-Term) antibody

Details for Product No. ABIN500614
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Antigen
Synonyms C9orf12, RGD1311271, rIpk1, RIP, RIP1, D330015H01Rik, Rinp, Rip1, 2400006N03Rik, RP23-83I13.5, Rip
Epitope
N-Term
(14), (7), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(74), (37), (37), (25), (23)
Host
Rabbit
(61), (11), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(52), (21), (19), (18), (16), (10), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN500614
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Immunogen RIPK1 antibody was raised against a 15 amino acid peptide from near the amino terminus of human RIPK1.
Isotype IgG
Specificity This antibody detects RIP.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name RIP
Background RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.Synonyms: Cell death protein RIP, RIPK1, Receptor-Interacting Protein, Receptor-interacting serine/threonine-protein kinase 1, Serine/threonine-protein kinase RIP
Gene ID 8737
NCBI Accession NP_003795
UniProt Q13546
Research Area Cancer, Apoptosis/Necrosis
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Background publications Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).

Hsu, Huang, Shu et al.: "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex." in: Immunity, Vol. 4, Issue 4, pp. 387-96, 1996 (PubMed).

Meylan, Burns, Hofmann et al.: "RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation." in: Nature immunology, Vol. 5, Issue 5, pp. 503-7, 2004 (PubMed).

Festjens, Vanden Berghe, Cornelis et al.: "RIP1, a kinase on the crossroads of a cell's decision to live or die." in: Cell death and differentiation, Vol. 14, Issue 3, pp. 400-10, 2007 (PubMed).

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