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TANK antibody (TRAF Family Member-Associated NFKB Activator) (N-Term)

Details for Product anti-TANK Antibody No. ABIN500869, Supplier: Log in to see
Antigen
  • C86182
  • E430026L09Rik
  • I-TRAF
  • ITRAF
  • TRAF2
Alternatives
anti-Human TANK antibody for Western Blotting
Epitope
N-Term
23
21
15
13
10
5
4
2
2
1
1
1
1
1
Reactivity
Human
115
38
36
5
3
2
1
1
1
1
Host
Rabbit
111
3
2
1
1
Clonality
Polyclonal
Conjugate
This TANK antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
102
39
38
22
15
13
11
11
10
2
2
1
Supplier
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Immunogen TANK antibody was raised against a 14 amino acid peptide from near the amino terminus of human TANK.
Isotype IgG
Specificity This antibody detects TANK / ITRAF at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Peptide affinity chromatography
Alternative Name TANK / ITRAF (TANK Antibody Abstract)
Background TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-kappaB. This activation of NF-kappaB occurs through an association with the kinases IKKepsilon and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IkappaB complex. This suggests that in addition to the possibility that TBK1 and IKKepsilon activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-kappaB proteins. At least two isoforms of TANK are known to exist.Synonyms: I-TRAF, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, TRAF2
Gene ID 10010
NCBI Accession NP_004171
Pathways p53 Signaling, TLR Signaling
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-TANK antibody (TRAF Family Member-Associated NFKB Activator) (N-Term) (ABIN500869) Immunocytochemistry of TANK in Daudi cells with this product at 2.5 μg/ml.
Western Blotting (WB) image for anti-TANK antibody (TRAF Family Member-Associated NFKB Activator) (N-Term) (ABIN500869) Western blot analysis of TANK in Daudi cell lysate with this product at (A) 0.5 and (...
Background publications Rao, Kadesch: "The intracellular form of notch blocks transforming growth factor beta-mediated growth arrest in Mv1Lu epithelial cells." in: Molecular and cellular biology, Vol. 23, Issue 18, pp. 6694-701, 2003 (PubMed).

Chariot, Leonardi, Muller, Bonif, Brown, Siebenlist: "Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37029-36, 2002 (PubMed).

Pomerantz, Baltimore: "NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase." in: The EMBO journal, Vol. 18, Issue 23, pp. 6694-704, 2000 (PubMed).

Cheng, Baltimore: "TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation." in: Genes & development, Vol. 10, Issue 8, pp. 963-73, 1996 (PubMed).

Rothe, Xiong, Shu, Williamson, Goddard, Goeddel: "I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8241-6, 1996 (PubMed).