E2F Transcription Factor 2 (E2F2) (Middle Region) antibody

Details for Product No. ABIN501674
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Antigen
Synonyms CG1071, Dmel\\CG1071, E2F, E2F2, dE2F-2, dE2F2, dE2f2, de2f2, e2f2, E2F-2, 9230110J10, E130207A07
Epitope
Middle Region
(12), (9), (8), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Cow (Bovine), Xenopus laevis, Human
(70), (38), (19), (12), (12), (12), (1)
Host
Rabbit
(62), (9), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(58), (20), (10), (5), (4), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN501674
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human E2F2
Sequence DQFLSPTLAC SSPLISFSPS LDQDDYLWGL EAGEGISDLF DSYDLGDLLI
Predicted Reactivity Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Bovine : 90 %, Guinea pig : 70 %
Characteristics This is a rabbit polyclonal antibody against E2F2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name E2F2
Background E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.
Molecular Weight 47 kDa
Gene ID 1870
NCBI Accession NP_004082, NM_004091
UniProt Q14209
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 437 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-E2F Transcription Factor 2 (E2F2) (Middle Region) antibody Immunohistochemistry with Brain, cerebellum tissue at an antibody concentration of 5µg/ml using anti-E2F2 antibody (ARP38418_P050)
Background publications Raj, Liu, Samadashwily et al.: "Survivin repression by p53, Rb and E2F2 in normal human melanocytes." in: Carcinogenesis, Vol. 29, Issue 1, pp. 194-201, 2008 (PubMed).

Validation Images
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