Spectrin alpha Chain, Brain (SPTAN1) (N-Term) antibody

Details for Product No. ABIN504492
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Antigen
Synonyms im:7157190, wu:fa20e05, wu:fb33g08, wu:fk32a10, zgc:112229, 2610027H02Rik, Spna-2, Spna2, EIEE5, NEAS, SPTA2, A2a, IPF, SPECA
Epitope
N-Term
(5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Zebrafish (Danio rerio), Fruit Fly (Drosophila melanogaster), Human, Chicken
(65), (26), (25), (15), (12), (9), (2), (1), (1), (1), (1)
Host
Rabbit
(74), (13), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(76), (40), (29), (28), (26), (21), (14), (10), (5), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN504492
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human SPTAN1
Sequence MDPSGVKVLE TAEDIQERRQ QVLDRYHRFK ELSTLRRQKL EDSYRFQFFQ
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against SPTAN1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SPTAN1
Background Fodrin (SPTAN1), which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Molecular Weight 272 kDa
Gene ID 6709
NCBI Accession NM_003127, NP_003118
UniProt Q13813
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 2472 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Spectrin alpha Chain, Brain (SPTAN1) (N-Term) antibody WB Suggested Anti-SPTAN1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Hela cell lysate
anti-Spectrin alpha Chain, Brain (SPTAN1) (N-Term) antibody (2) Immunohistochemistry with Kidney tissue at an antibody concentration of 5µg/ml using anti-SPTAN1 antibody (ARP58532_P050)
anti-Spectrin alpha Chain, Brain (SPTAN1) (N-Term) antibody (3) Sample Type: NT2 cells
Red: Antibody
Blue: DAPI
Primary Dilution: 1ug/50ul antibody
Secondary Antibody: Alexa goat anti-rabbit 594
anti-Spectrin alpha Chain, Brain (SPTAN1) (N-Term) antibody (4) Immunohistochemistry with NT-2 cell line, mouse brain, and rat brain tissue
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Validation Images
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