EBF1 antibody (Internal Region)
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- Target See all EBF1 Antibodies
- EBF1 (Early B-Cell Factor 1 (EBF1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This EBF1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-HNQLPALANT SV
- Specificity
- This antibody detects EBF1 / COE1 (Internal region). It is expected not to cross-react with EBF2, EBF3 and EBF4.
- Cross-Reactivity (Details)
- Species reactivity (expected):Mouse, ratSpecies reactivity (tested):Human
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from the internal region of the protein sequence according to NP_076870.1
- Top Product
- Discover our top product EBF1 Primary Antibody
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- Application Notes
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Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-0.1 μg/mL, approx.75 kDa band observed in Human Adipose lysates. Anonymous customer used this product successfully in IP from induced 3T3-L1 expressingFLAG-EBF1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.
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- Target
- EBF1 (Early B-Cell Factor 1 (EBF1))
- Alternative Name
- EBF1 / COE1 (EBF1 Products)
- Synonyms
- EBF1 antibody, 9830166E18Rik antibody, AW990631 antibody, Ebf antibody, O/E-1 antibody, Olf-1 antibody, Olf1 antibody, COE1 antibody, EBF antibody, OLF1 antibody, early B-cell factor 1 antibody, early B cell factor 1 antibody, EBF1 antibody, Ebf1 antibody
- Background
- EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.Synonyms: EBF, Early B-cell factor, OE-1, Transcription factor COE1
- Gene ID
- 1879
- NCBI Accession
- NP_076870
- UniProt
- Q9UH73
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