Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody

Antigen: Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2)

Synonyms: PDHK2, 3j828, MGC52849, MGC132353, Pdk, MGC56209, zgc:56209, PDK2

Binding Site: AA 187-276

Clonality: Monoclonal (2G1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA, RNA Interference (RNAi)

Certificates: ISO 9001:2008

Catalog no.: ABIN562160

Additional Information

Alternative name: PDK2

Gene ID: 5164, BC005811

Immunogen: PDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: HIGSIDPNCNVSEVVKDAYD MAKLLCDKYYMASPDLEIQE INAANSKQPIHMVYVPSHLY HMLFELFKNAMRATVESHES SLILPPIKVM

Cross-Reactivity: Human

Isotype: IgG2a kappa

Clone: 2G1

Description: Gene: pyruvate dehydrogenase kinase, isozyme 2
Synonyms: PDHK2

Characteristics: Mouse monoclonal antibody raised against a partial recombinant PDK2.

Application Details

Application Notes: Application specialization: Cell lysate, Recombinant protein, Transfected lysate

Buffer: 1x PBS, pH 7.2

Storage: -20 °C

Storage Comment: Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

Research Area: Cancer, Metabolism

Restrictions: For Research Use only

Images

PDK2 monoclonal antibody (M01), clone 2G1 Western Blot analysis of PDK2 expression in U-2 OS

Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody (M01) clone 2G1 GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8 ug/ml]

Detection limit for recombinant GST tagged PDK2 is approximately 0.3ng/ml as a capture antibody.

Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody (M01), clone 2G1.

Lane 1: PDK2 transfected lysate(46.2 KDa).
Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (35.31 KDa) .

Publications

Product: Ohlsson, Karlsson, Edlund: "IPF1, a homeodomain-containing transactivator of the insulin gene." in: The EMBO journal, Vol. 12, Issue 11, pp. 4251-9, 1993 (PubMed).

Gu, Dubauskaite, Melton: "Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors." in: Development (Cambridge, England), Vol. 129, Issue 10, pp. 2447-57, 2002 (PubMed).

Babu, Deering, Mirmira: "A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis." in: Molecular genetics and metabolism, Vol. 92, Issue 1-2, pp. 43-55, 2007 (PubMed).

Gannon, Ables, Crawford et al.: "pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis." in: Developmental biology, Vol. 314, Issue 2, pp. 406-17, 2008 (PubMed).