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Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody

Details for Product No. ABIN562160
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Antigen
Synonyms PDHK2, PDKII, 3j828, MGC52849, MGC132353, Pdk, zgc:56209, PDK2
Epitope
AA 187-276
(11), (8), (8), (7), (6), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(78), (45), (31), (13), (12), (12), (2)
Host
Mouse
(66), (23)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA, RNA Interference (RNAi)
(80), (52), (32), (19), (10), (10), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 11 to 12 Business Days
Catalog No. ABIN562160
394.24 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen PDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: HIGSIDPNCNVSEVVKDAYD MAKLLCDKYYMASPDLEIQE INAANSKQPIHMVYVPSHLY HMLFELFKNAMRATVESHES SLILPPIKVM
Clone 2G1
Isotype IgG2a kappa
Characteristics Mouse monoclonal antibody raised against a partial recombinant PDK2.
Alternative Name PDK2
Background Gene: pyruvate dehydrogenase kinase, isozyme 2
Synonyms: PDHK2
Gene ID 5164, BC005811
Research Area Cancer, Metabolism
Application Notes Application specialization: Cell lysate, Recombinant protein, Transfected lysate
Restrictions For Research Use only
Buffer 1x PBS, pH 7.2
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Supplier Images
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody PDK2 monoclonal antibody (M01), clone 2G1 Western Blot analysis of PDK2 expression in U-2 OS (ABIN1339563).
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody (2) Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody (M01) clone 2G1 (ABIN562160). GAPDH (36.1 kDa) used as specificity and loading control.
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody (3) Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8 µg/mL]
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody (4) Detection limit for recombinant GST tagged PDK2 is approximately 0.3 ng/mL as a capture antibody.
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody (5) Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody (M01), clone 2G1.

Lane 1: PDK2 transfected lysate(46.2 kDa).
Lane 2: Non-transfected lysate.
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody (6) Western Blot detection against Immunogen (35.31 kDa) .
anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (AA 187-276) antibody (7) Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8 µg/mL]
Product cited in: Ohlsson, Karlsson, Edlund: "IPF1, a homeodomain-containing transactivator of the insulin gene." in: The EMBO journal, Vol. 12, Issue 11, pp. 4251-9, 1993 (PubMed).

Gu, Dubauskaite, Melton: "Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors." in: Development (Cambridge, England), Vol. 129, Issue 10, pp. 2447-57, 2002 (PubMed).

Babu, Deering, Mirmira: "A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis." in: Molecular genetics and metabolism, Vol. 92, Issue 1-2, pp. 43-55, 2007 (PubMed).

Gannon, Ables, Crawford et al.: "pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis." in: Developmental biology, Vol. 314, Issue 2, pp. 406-17, 2008 (PubMed).

Hosts (66), (23)
Reactivities (78), (45), (31), (13), (12), (12), (2)
Applications (80), (52), (32), (19), (10), (10), (2), (2), (1), (1)
Conjugates (5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (11), (8), (8), (7), (6), (4), (3), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN562160
394.24 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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