Zyxin (ZYX) (AA 1-572) antibody

Details for Product No. ABIN563471
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Antigen
Synonyms ESP-2, HED-2, R75157, 9530098H06Rik, BcDNA:LD06023, CG32018, DLim-1, DmLIM-1, Dmel\\CG32018, Lim102EF, ZYX102, Zyx102, Zyx102EF, lim, zyx102, zyxin, zgc:171680, ZYX, zyx, MGC145270
Epitope
AA 1-572
(17), (12), (5), (5), (3), (3), (2), (1), (1), (1)
Reactivity
Human
(71), (36), (33), (26), (25), (24), (12), (1)
Host
Mouse
(57), (11), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunoprecipitation (IP), RNA Interference (RNAi)
(53), (31), (20), (15), (10), (8), (8), (4), (3), (2)
Pubmed 1 reference available
Catalog no. ABIN563471
Quantity 100 µg
Price
343.81 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: MAAPRPSPAISVSVSAPAFY APQEKFGPVVAPKPKVNPFR PGDSEPPPAPGAQRAQMGRV GEIPPPPPEDFPLPPPPLAG DGDDAEGALGGAFPPPPPPI EESFPPAPLEEEIFPSPPPP PEEEGGPEAPIPPPPQPREK VSSIDLEIDSLSSLLDDMTK NDPFKARVSSGYVPPPVATP FSSKSSTKPAAGGTAPLPPW KSPSSSQPLPQVPAPAQSQT QFHVQPQPQPKPQVQLHVQS QTQPVSLANTQPRGPPASSP APAPKFSPVTPKFTPVASKF SPGAPGGSGSQPNQKLGHPE ALSAGTGSPQPPSFTYAQQR EKPRVQEKQHPVPPPAQNQN QVRSPGAPGPLTLKEVEELE QLTQQLMQDMEHPQRQNVAV NELCGRCHQPLARAQPAVRA LGQLFHIACFTCHQCAQQLQ GQQFYSLEGAPYCEGCYTDT LEKCNTCGEPITDRMLRATG KAYHPHCFTCVVCARPLEGT SFIVDQANRPHCVPDYHKQY APRCSVCSEPIMPEPGRDET VRVVALDKNFHMKCYECEDC GKPLSIEADDNGCFPLDGHV LCRKCHTARAQT
Clone 2C10-4A7
Isotype IgG1 kappa
Characteristics Mouse monoclonal antibody raised against a full length recombinant ZYX.
Alternative Name Zyxin
Background Gene: zyxin
Synonyms: ESP-2, HED-2
Gene ID 7791, BC008743
Application Notes Application specialization: Recombinant protein, Transfected lysate
Restrictions For Research Use only
Buffer 1x PBS, pH 7.2
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Supplier Images
anti-Zyxin (ZYX) (AA 1-572) antibody Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.

Lane 1: ZYX transfected lysate(61.3 kDa).
Lane 2: Non-transfected lysate.
anti-Zyxin (ZYX) (AA 1-572) antibody (2) Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (ABIN563471). GAPDH (36.1 kDa) used as specificity and loading control.
anti-Zyxin (ZYX) (AA 1-572) antibody (3) Immunofluorescence of monoclonal antibody to ZYX on HeLa cell. [antibody concentration 10 µg/mL]
anti-Zyxin (ZYX) (AA 1-572) antibody (4) Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.
anti-Zyxin (ZYX) (AA 1-572) antibody (5) Detection limit for recombinant GST tagged ZYX is 1 ng/mL as a capture antibody.
anti-Zyxin (ZYX) (AA 1-572) antibody (6) Western Blot detection against Immunogen (88.66 KDa) .
anti-Zyxin (ZYX) (AA 1-572) antibody (7) Immunofluorescence of monoclonal antibody to ZYX on HeLa cell. [antibody concentration 10 µg/mL]
Product cited in: Hirata, Tatsumi, Sokabe: "Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner." in: Journal of cell science, Vol. 121, Issue Pt 17, pp. 2795-804, 2008 (PubMed).

Hosts (57), (11), (3)
Reactivities (71), (36), (33), (26), (25), (24), (12), (1)
Applications (53), (31), (20), (15), (10), (8), (8), (4), (3), (2)
Conjugates (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (17), (12), (5), (5), (3), (3), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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