Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (full length) antibody

Details for Product No. ABIN565913
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Antigen
Synonyms 5730484M20Rik, C230082M18, RNF116, RNF196, fc43f10, si:dkey-69h6.7, wu:fc43f10
Epitope
full length
(27), (14), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(69), (27), (27), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(47), (16), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, RNA Interference (RNAi)
(44), (39), (22), (10), (8), (3)
Pubmed 7 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 11 to 12 Business Days
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Immunogen CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 kDa.
Immunogen sequence: MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY ESLSEKQGMT QESFDTSGAG AGRGADPRVP PSSPATQVCF EEPQPSTSTS DLFPTASASS TEPSPAGRER SSSCGSGGGG ISPKGSGPSV ASDEVSSFAS ALPDRKTASF SSLEPQDQED LEPVKKKMRG DGDLDLNGQL LVAQPRRNAQ TVHEDVRAAA GKPDKMEETL TCIICQDLLH DCVSLQPCMH TFCAACYSGW MERSSLCPTC RCPVERICKN HILNNLVEAY LIQHPDKSRS EEDVQSMDAR NKITQDMLQP KVRRSFSDEE GSSEDLLELS DVDSESSDIS QPYVVCRQCP EYRRQAAQPP HCPAPEGEPG APQALGDAPS TSVSLTTAVQ DYVCPLQGSH ALCTCCFQPM PDRRVEREQD PRVAPQQCAV CLQPFCHLYW GCTRTGCYGC LAPFCELNLG DKCLDGVLNN NSYESDILKN YLATRGLTWK NMLTESLVAL QRGVFLLSDY RVTGDTVLCY CCGLRSFREL TYQYRQNIPA SELPVAVTSR PDCYWGRNCR TQVKAHHAMK FNHICEQTRF KN
Clone 1H3-A12
Isotype IgG1 kappa
Alternative Name CHFR (CHFR Antibody Abstract)
Background Gene: checkpoint with forkhead and ring finger domains
Synonyms: FLJ10796, FLJ33629, RNF116, RNF196
Gene ID 55743
Research Area Cancer, Cell Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20
Storage Comment Store at -20
Supplier Images
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (full length) antibody Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded h...
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (full length) antibody (2) Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR V...
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (full length) antibody (3) Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/mL as a captu...
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (full length) antibody (4) Western Blot detection against Immunogen (97.46 KDa) .
Product cited in: Kashima, Idogawa, Mita et al.: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, 2012 (PubMed).

Fujita, Yamamoto, Imamura et al.: "Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach." in: Virchows Archiv : an international journal of pathology, Vol. 460, Issue 2, pp. 163-9, 2012 (PubMed).

Hu, Huang, Sun et al.: "Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 447-54, 2011 (PubMed).

Gao, Xin, Zhang et al.: "Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma." in: World journal of gastroenterology : WJG, Vol. 14, Issue 32, pp. 5000-7, 2008 (PubMed).

Privette, González, Ding et al.: "Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression." in: Cancer research, Vol. 67, Issue 13, pp. 6064-74, 2007 (PubMed).

Background publications Privette, Weier, Nguyen et al.: "Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 7, pp. 643-52, 2008 (PubMed).

Milne, Sitarz, Carvalho et al.: "Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis." in: Human pathology, Vol. 38, Issue 6, pp. 903-13, 2007 (PubMed).

Validation Images
Catalog No. ABIN565913
330.00 $
Plus shipping costs $45.00 and $22.00 dry ice
Quantity
Price
100 μg
330.00 $

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