Caspase 4 antibody (Middle Region)

Catalog No. ABIN567448

This Rabbit Polyclonal antibody specifically detects Caspase 4 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for Caspase 4 antibody (Middle Region) (ABIN567448)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 4 Antibody

Binding Specificity: Middle Region

Sequence: GILEGICGTV HDEKKPDVLL YDTIFQIFNN RNCLSLKDKP KVIIVQACRG

Predicted Reactivity: Human: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CASP4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CASP4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 377 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: CASP4

Background: This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
Alias Symbols: ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX
Protein Interaction Partner: MYH9, ELF4, APP, TRAF6, PLA2G4A, NOD1, CASP14, CASP4, APAF1, SERPINB9, CASP10, IL18, CASP3, IL1B, DSP, PSEN1, IL37, CASP8, CASP1, XIAP, MYCBPAP, SLC25A22, SRPRB, CTNNBIP1, DNAAF5, MTHFD1L, UFL1, GCN1L1, SMC2, NUP93, SMC3, SMC1A, USP9X, VDAC3, PSMB1, MSH6,
Protein Size: 377

Molecular Weight: 10 kDa

Gene ID: 837

NCBI Accession: NM_001225, NP_001216

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity