FEN1 antibody (C-Term)
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- Target See all FEN1 Antibodies
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Dog
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FEN1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- FEN1 antibody was raised against the C terminal of FEN1
- Purification
- Purified
- Immunogen
- FEN1 antibody was raised using the C terminal of FEN1 corresponding to a region with amino acids PNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSL
- Top Product
- Discover our top product FEN1 Primary Antibody
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- Application Notes
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WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator. - Comment
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FEN1 Blocking Peptide, catalog no. 33R-7232, is also available for use as a blocking control in assays to test for specificity of this FEN1 antibody
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of FEN1 antibody in PBS
- Concentration
- Lot specific
- Buffer
- PBS
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- Target
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Alternative Name
- FEN1 (FEN1 Products)
- Synonyms
- FEN-1 antibody, FEN1 antibody, AW538437 antibody, MF1 antibody, RAD2 antibody, cb879 antibody, fen-1 antibody, fen1 antibody, mf1 antibody, rad2 antibody, xFEN1a antibody, flap structure-specific endonuclease 1 antibody, flap endonuclease 1-like antibody, Flap endonuclease-1 antibody, 5'-3' exonuclease family protein antibody, flap structure specific endonuclease 1 antibody, flap structure-specific endonuclease 1 L homeolog antibody, nuclease, Rad2 family antibody, FEN1 antibody, LOC100808044 antibody, Bm1_49605 antibody, AT5G26680 antibody, Fen1 antibody, fen1 antibody, fen1.L antibody
- Background
- FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
- Molecular Weight
- 42 kDa (MW of target protein)
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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