CD14 antibody (CD14 Molecule)

Details for Product anti-CD14 Antibody No. ABIN638430, Supplier: Log in to see
Antigen
  • CD14 molecule
  • CD14 antigen
  • CD14
  • Cd14
Alternatives
anti-Human CD14 antibody for Functional Studies
Reactivity
Human, Non-Human Primate
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This CD14 antibody is un-conjugated
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Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Clone MEM-18
Isotype IgG1
Specificity The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
HLDA III, WS Code M 253
HLDA IV, WS Code M 314
HLDA V, WS Code M MA087
HLDA VI, WS Code M MA95
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Alternative Name CD14 (CD14 Antibody Abstract)
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Gene ID 929
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-CD14 Molecule (CD14) antibody (ABIN638430) Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 usin...
Flow Cytometry (FACS) image for anti-CD14 Molecule (CD14) antibody (ABIN638430) Surface staining of human peripheral blood leukocytes using anti-human CD14
Product cited in: Weiss, Lichtenauer, Kirchner, Stock, Aurich, Christ, Brockhoff, Kunz-Schughart, Jauch, Schlitt, Thasler: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Iwaki, Nishitani, Mitsuzawa, Hyakushima, Sano, Kuroki: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005 (PubMed).

Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, 2002 (PubMed).

Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, 2001 (PubMed).

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

Bazil, Baudys, Hilgert, Stefanová, Low, Zbrozek, Horejsí: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, 1989 (PubMed).

Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, 1987 (PubMed).

Background publications Lødrup Carlsen, Granum: "Soluble CD14: role in atopic disease and recurrent infections, including otitis media." in: Current allergy and asthma reports, Vol. 7, Issue 6, pp. 436-43, 2007 (PubMed).

Asai, Makimura, Kawabata, Ogawa: "Soluble CD14 discriminates slight structural differences between lipid as that lead to distinct host cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 11, pp. 7674-83, 2007 (PubMed).

Fernández-Real, Broch, Richart, Vendrell, López-Bermejo, Ricart: "CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity." in: The Journal of clinical endocrinology and metabolism, Vol. 88, Issue 4, pp. 1780-4, 2003 (PubMed).

Juan, Hailman, Kelley, Wright, Lichenstein: "Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17237-42, 1995 (PubMed).

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