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CD14 antibody (CD14 Molecule)

Details for Product anti-CD14 Antibody No. ABIN638430, Supplier: Log in to see
Antigen
Alternatives
anti-Human CD14 antibody for Functional Studies
Reactivity
Human, Non-Human Primate
664
101
46
29
29
26
24
24
22
20
20
19
19
12
12
11
9
9
5
4
3
2
1
Host
Mouse
595
99
47
24
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD14 antibody is un-conjugated
105
89
63
49
11
11
11
9
8
7
6
6
5
5
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
577
178
149
120
114
73
50
43
30
18
14
13
11
4
4
3
3
3
3
2
2
2
1
1
1
1
1
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Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Clone MEM-18
Isotype IgG1
Specificity The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64. HLDA III, WS Code M 253HLDA IV, WS Code M 314HLDA V, WS Code M MA087HLDA VI, WS Code M MA95
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Alternative Name CD14 (CD14 Antibody Abstract)
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Gene ID 929
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Pathways TLR Signaling
Application Notes Flow Cytometry: Recommended dilution: 4 µg/mL
Western Blotting:
Application note: Non-reducing conditions.
ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 -, labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-CD14 antibody (CD14 Molecule) (ABIN638430) Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 usin...
Flow Cytometry (FACS) image for anti-CD14 antibody (CD14 Molecule) (ABIN638430) Surface staining of human peripheral blood leukocytes using anti-human CD14
Product cited in: Weiss, Lichtenauer, Kirchner, Stock, Aurich, Christ, Brockhoff, Kunz-Schughart, Jauch, Schlitt, Thasler: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007

Iwaki, Nishitani, Mitsuzawa, Hyakushima, Sano, Kuroki: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005

Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, 2002

Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, 2001

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001

Bazil, Baudys, Hilgert, Stefanová, Low, Zbrozek, Horejsí: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, 1989

Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, 1987

Background publications Lødrup Carlsen, Granum: "Soluble CD14: role in atopic disease and recurrent infections, including otitis media." in: Current allergy and asthma reports, Vol. 7, Issue 6, pp. 436-43, 2007

Asai, Makimura, Kawabata, Ogawa: "Soluble CD14 discriminates slight structural differences between lipid as that lead to distinct host cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 11, pp. 7674-83, 2007

Fernández-Real, Broch, Richart, Vendrell, López-Bermejo, Ricart: "CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity." in: The Journal of clinical endocrinology and metabolism, Vol. 88, Issue 4, pp. 1780-4, 2003

Juan, Hailman, Kelley, Wright, Lichenstein: "Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17237-42, 1995