CASP5 antibody

Catalog No. ABIN643987

Product Details anti-CASP5 Antibody

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP5 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit Anti-Human CASP5

Specificity: DS-PB-00380 recognizes an epitope located within the internal region of human Caspase5, a 47kDa cysteine protease, otherwise known as ICH3. Caspase5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase5 is a member of a growing number of inflammatory caspases belonging to the Caspase1 (ICEtype) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase1, Caspase5 is not directly responsible for the activation of IL1Beta from the proIL1Beta precursor, but the presence of Caspase5 does appear to enhance the processing of IL1Beta. The in vivo expression of Caspase5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)induced apoptosis (1).

Characteristics: Phosphorylation/Apoptosis/Signal Transduction/Regulation

Purification: Purified

Immunogen: Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Isotype: IgG

Application Details

Application Notes: Western Blotting

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 1.0 mg/ml

Buffer: Phosphate buffered saline

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for CASP5

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Alternative Name: Caspase-5 (CASP5 Products)

Synonyms: ICE(rel)III antibody, ICEREL-III antibody, ICH-3 antibody, CASP5 antibody, caspase 5 antibody, caspase-5 antibody, CASP5 antibody, LOC100437308 antibody

Pathways: Inflammasome