NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41)

Details for Product anti-NFKBIA Antibody No. ABIN652417, Supplier: Log in to see
Antigen
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • LOC100220015
  • LOC100357341
  • Nfkbia
Alternatives
anti-Human NFKBIA antibody for Western Blotting
Epitope
AA 12-41, Ser32, Ser36
73
53
52
40
25
23
22
20
18
15
15
14
14
11
5
5
5
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
508
263
216
49
40
29
19
11
2
2
2
1
1
Host
Rabbit
349
166
6
Clonality (Clone)
Polyclonal ()
Conjugate
This NFKBIA antibody is un-conjugated
19
15
14
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
395
182
178
88
87
84
68
40
34
19
9
6
5
4
3
3
2
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 AA from human NFKBIA.
Clone RB21911
Isotype Ig
Specificity This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids from human NFKBIA.
Predicted Reactivity Mouse (Murine),Pig (Porcine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NFKBIA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Molecular Weight 35478 DA
Gene ID 4792
UniProt P25963
Research Area Apoptosis/Necrosis, Cell Signaling, Transcription Factors, Cell Structure
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 12-41), (Ser32), (Ser36) antibody (ABIN652417) Western blot analysis of NFKBIA-S32/36 (ABIN652417) in Hela cell line lysates (35 µg/...
Immunohistochemistry (IHC) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 12-41), (Ser32), (Ser36) antibody (ABIN652417) Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/...
Flow Cytometry (FACS) image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 12-41), (Ser32), (Ser36) antibody (ABIN652417) Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histog...
Background publications Tojima, Fujimoto, Delhase, Chen, Hatakeyama, Nakayama, Kaneko, Nimura, Motoyama, Ikeda, Karin, Nakanishi: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000 (PubMed).

Gil, Alcamí, Esteban: "Activation of NF-kappa B by the dsRNA-dependent protein kinase, PKR involves the I kappa B kinase complex." in: Oncogene, Vol. 19, Issue 11, pp. 1369-78, 2000 (PubMed).

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