NLRP3 antibody (N-Term)

Catalog No. ABIN652504

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: AA 65-91, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NLRP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-91 amino acids from the N-terminal region of human NLRP3.

Clone: RB22404

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000-1:2000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-NLRP3 antibody (ABIN652504)

Liu, Wen, Chen, Wang, Huang, Tu: "Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: BioMed research international, Vol. 2016, pp. 2503703, (2016) (PubMed).

Jhang, Lu, Ho, Cheng, Yen: "Protective Effects of Catechin against Monosodium Urate-Induced Inflammation through the Modulation of NLRP3 Inflammasome Activation." in: Journal of agricultural and food chemistry, (2015) (PubMed).

Liu, Tu, Chen, Wang, Hu, Dong: "Effects of Modified Simiao Decoction on IL-1 β and TNF α Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 406816, (2014) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3 (NLRP3 Products)

Synonyms: CIAS1 antibody, Nalp3 antibody, avp antibody, cias1 antibody, nalp3 antibody, pypaf1 antibody, agtavprl antibody, AGTAVPRL antibody, AII antibody, AVP antibody, C1orf7 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, MWS antibody, NALP3 antibody, PYPAF1 antibody, AII/AVP antibody, Cias1 antibody, Mmig1 antibody, Pypaf1 antibody, NLR family pyrin domain containing-like antibody, NLR family pyrin domain containing 3 antibody, NLR family, pyrin domain containing 3 L homeolog antibody, NLR family, pyrin domain containing 3 antibody, NLRPL antibody, NLRP3 antibody, nlrp3.L antibody, Nlrp3 antibody

Background: NLRP3 is a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis.

Molecular Weight: 118173

Gene ID: 114548

NCBI Accession: NP_001073289, NP_001120933, NP_001120934, NP_001230062, NP_004886, NP_899632

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome