Retinoblastoma 1 (RB1) (C-Term), (AA 858-886) antibody

Details for Product No. ABIN652516
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms RB1, OSRC, RB, p105-Rb, pRb, pp110, Rb, Rb-1, zgc:154147, P105-RB, PP110, RB11
Epitope
C-Term, AA 858-886
(34), (33), (29), (22), (19), (19), (18), (17), (17), (15), (12), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(355), (227), (215), (109), (85), (65), (48), (7), (1), (1), (1)
Host
Rabbit
(314), (54), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(226), (100), (98), (94), (82), (41), (37), (18), (14), (11), (2), (1)
Pubmed 2 references available
Quantity 400 μL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN652516
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 AA from the C-terminal region of human RB1.
Clone RB20715
Isotype Ig
Specificity This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 865~894 amino acids from the C-terminal region of human RB1
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name RB1
Background RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Synonyms: Retinoblastoma-associated protein, pRb, Rb, pp110, p105-Rb, RB1
Molecular Weight 106159 DA
Gene ID 5925
UniProt P06400
Research Area Cancer, Transcription Factors, Signaling, Neurology, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Retinoblastoma 1 (RB1) (C-Term), (AA 858-886) antibody Western blot analysis of RB1 Antibody (C-term) (ABIN652516) in 293 cell line lysates (35 µg/lane). RB1 (arrow) was detected using the purified polyclonal antibody.(2 µg/mL)
anti-Retinoblastoma 1 (RB1) (C-Term), (AA 858-886) antibody (2) Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Retinoblastoma 1 (RB1) (C-Term), (AA 858-886) antibody (3) RB1 Antibody (C-term) (ABIN652516) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).

Connell-Crowley, Harper, Goodrich: "Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 287-301, 1997 (PubMed).

Validation Images
Did you look for something else?
back to top