Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1) (C-Term), (AA 923-951) antibody

Details for Product No. ABIN652666
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Antigen
Epitope
C-Term, AA 923-951
(21), (7), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (36), (26)
Host
Rabbit
(62), (3), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(58), (29), (14), (10), (9), (7), (5), (4), (3), (3), (3), (2), (2)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN652666
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Immunogen This CARD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-951 AA from the C-terminal region of human CARD4.
Clone RB21998
Isotype Ig
Specificity This CARD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 924~953 amino acids from the C-terminal region of human CARD4.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CARD4
Background CARD4 is a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.
Synonyms: Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, CARD4, NOD1
Molecular Weight 107691 DA
Gene ID 10392
UniProt Q9Y239
Research Area Apoptosis/Necrosis
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.41 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1) (C-Term), (AA 923-951) antibody Western blot analysis of CARD4 Antibody (C-term) (ABIN652666) in HL-60 cell line lysates (35 µg/lane). CARD4 (arrow) was detected using the purified polyclonal antibody.
anti-Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1) (C-Term), (AA 923-951) antibody (2) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CARD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1) (C-Term), (AA 923-951) antibody (3) CARD4 Antibody (C-term) (ABIN652666) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Inohara, Koseki, del Peso et al.: "Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14560-7, 1999 (PubMed).

Inohara, Koseki, Lin et al.: "An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27823-31, 2000 (PubMed).

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