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Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (AA 950-979), (C-Term) antibody

Details for Product No. ABIN654447, Supplier: Login to see
Antigen
  • 75kDa
  • AW260155
  • INPP5P
  • 5PTase
Epitope
AA 950-979, C-Term
15
15
4
2
2
2
1
Reactivity
Human
34
6
5
1
Host
Rabbit
31
3
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
32
19
18
13
4
1
1
1
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Immunogen This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 950-979 AA from the C-terminal region of human INPP5B.
Clone RB27636
Isotype Ig
Specificity This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 957-987 amino acids from the C-terminal region of human INPP5B.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name INPP5B (INPP5B Antibody Abstract)
Background Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].
Synonyms: INPP5B,Type II inositol-1,4,5-trisphosphate 5-phosphatase,
Molecular Weight 112852 DA
Gene ID 3633
NCBI Accession NP_005531
UniProt P32019
Research Area Metabolism, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (AA 950-979), (C-Term) antibody (ABIN654447) INPP5B Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane)....
Immunohistochemistry (IHC) image for anti-Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (AA 950-979), (C-Term) antibody (ABIN654447) INPP5B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffi...
Flow Cytometry (FACS) image for anti-Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (AA 950-979), (C-Term) antibody (ABIN654447) INPP5B Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) com...
Background publications Coon, Mukherjee, Hanna et al.: "Lowe syndrome patient fibroblasts display Ocrl1-specific cell migration defects that cannot be rescued by the homologous Inpp5b phosphatase." in: Human molecular genetics, Vol. 18, Issue 23, pp. 4478-91, 2009 (PubMed).

Mao, Balkin, Zoncu et al.: "A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism." in: The EMBO journal, Vol. 28, Issue 13, pp. 1831-42, 2009 (PubMed).

Williams, Choudhury, McKenzie et al.: "Targeting of the type II inositol polyphosphate 5-phosphatase INPP5B to the early secretory pathway." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 3941-51, 2007 (PubMed).

Jänne, Rochelle, Martin-DeLeon et al.: "Mapping of the 75-kDa inositol polyphosphate-5-phosphatase (Inpp5b) to distal mouse chromosome 4 and its exclusion as a candidate gene for dysgenetic lens." in: Genomics, Vol. 28, Issue 2, pp. 280-5, 1996 (PubMed).