CYP1A2 antibody (AA 221-320) (HRP)

Catalog No. ABIN742479

The HRP-conjugated Rabbit Polyclonal anti-CYP1A2 antibody (ABIN742479) specifically detects CYP1A2 in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CYP1A2 antibody (AA 221-320) (HRP) (ABIN742479)

Target: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP1A2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CYP1A2 Antibody (HRP)

Binding Specificity: AA 221-320

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cytochrome P450 1A2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CYP1A2

Target: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Alternative Name: Cytochrome P450 1A2

Background:

Synonyms: CP12, P3-450, P450(PA), Cytochrome P450 1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3, CYP1A2

Background: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.

Gene ID: 1544

UniProt: P05177