CDK5 antibody

Catalog No. ABIN782438

The Mouse Monoclonal anti-CDK5 antibody has been validated for WB and IHC (fro). It is suitable to detect CDK5 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for CDK5 antibody (ABIN782438)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1H3

Product Details anti-CDK5 Antibody

Specificity: Specific for the ~ 28 kDa Cdk5 protein in Western blots of Rat striatal lysate. The antibody shows no cross reactivity with the CDK5 cofactor p35 or its degradation product p25.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Protein G Chromatography.

Immunogen: Purified Rat Cdk5.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

References for anti-CDK5 antibody (ABIN782438)

Amin, Zheng, Bk, Shukla, Skuntz, Grant, Steiner, Bhaskar, Pant: "The interaction of Munc 18 (p67) with the p10 domain of p35 protects in vivo Cdk5/p35 activity from inhibition by TFP5, a peptide derived from p35." in: Molecular biology of the cell, Vol. 27, Issue 21, pp. 3221-3232, (2017) (PubMed).

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background: The neuronal protein kinase, Cdk5 has been implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (Kansy J et al., 2004), the presynaptic vesicle cycle (Nguyen, C. & Bibb, JA 2003), neurotransmitter receptor trafficking and dopamine neurotransmission (Bibb, JA et al. 1999). At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer's, Parkinson's, Huntington's, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology.Synonyms: Cell division protein kinase 5, Cyclin-dependent kinase 5, PSSALRE, Serine/threonine-protein kinase PSSALRE, TPKII catalytic subunit, Tau protein kinase II catalytic subunit

Gene ID: 140908

NCBI Accession: NP_543161

UniProt: Q03114

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis