CDK5 antibody (C-Term)

Catalog No. ABIN3030614

The Rabbit Polyclonal anti-CDK5 antibody is suitable to detect CDK5 in samples from Human and Mouse. It has been validated for WB, ELISA, FACS and IF.

Quick Overview for CDK5 antibody (C-Term) (ABIN3030614)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-CDK5 Antibody

Binding Specificity: AA 254-289, C-Term

Purpose: CDK5 Antibody

Cross-Reactivity (Details): Expected species reactivity: Rat, Bovine, Xenopus

Purification: Antigen affinity

Immunogen: This CDK5 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 254-289 amino acids from the C-terminal region of human CDK5.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CDK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CDK5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background: Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity, synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity.[UniProt]

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis