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CD4 antibody

CD4 Reactivity: Human FACS Host: Mouse Monoclonal 7E14 unconjugated
Catalog No. ABIN793377
  • Target See all CD4 Antibodies
    CD4
    Reactivity
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    • 199
    • 113
    • 38
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    Human
    Host
    • 328
    • 146
    • 108
    • 4
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    • 1
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    Mouse
    Clonality
    • 445
    • 138
    • 3
    Monoclonal
    Conjugate
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    This CD4 antibody is un-conjugated
    Application
    • 470
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    Flow Cytometry (FACS)
    Specificity
    Human CD4
    Purification
    Protein A/G purified
    Immunogen
    Human peripheral blood T lymphocytes.
    Clone
    7E14
    Isotype
    IgG1
    Top Product
    Discover our top product CD4 Primary Antibody
  • Application Notes
    Approved: Flo

    Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, 0.08 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Aliquot to Avoid freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot to avoid freeze/thaw cycles.
  • Target
    CD4
    Alternative Name
    CD4 (CD4 Products)
    Synonyms
    cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
    Background
    Name/Gene ID: CD4

    Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule
    Gene ID
    920
    UniProt
    P01730
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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