CHEK2 antibody (N-Term)

Catalog No. ABIN927287

Product Details anti-CHEK2 Antibody

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Immunogen: CHEK2 antibody was raised in rabbit using the N terminal of CHEK2 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CHEK2 Blocking Peptide, catalog no. 33R-6486, is also available for use as a blocking control in assays to test for specificity of this CHEK2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2 (CHEK2 Products)

Synonyms: CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Synonyms: Polyclonal CHEK2 antibody, Anti-CHEK2 antibody, CHK2 checkpoint homolog, S. pombe antibody, RP11-436C9.1 antibody, CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody.

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle