HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (MEM-G-1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94366

Additional Information

Alternative name: HLA-G

Immunogen: Denatured bacterially expressed recombinant human HLA-G heavy chain.

Isotype: IgG1

Clone: MEM-G-1

Specificity: The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to theMHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface oftrophoblast cells.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 1: 60 to 1: 100Incubation: 1 h at RTPositive tissue: Human placenta - extravillous cytotrophoblastStaining technique: Standard ABC techniquePretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.

Publications

Fournel, Huc, Aguerre-Girr et al.: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).

Frumento, Franchello, Palmisano et al.: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, 2000 (PubMed).

Hurks, Valter, Wilson et al.: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, 2001 (PubMed).

Boyson, Erskine, Whitman et al.: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).

Shih, Kurman: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, 2004 (PubMed).

Gonen-Gross, Achdout, Arnon et al.: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Biondi, Ferretti, Lunghi et al.: "cAMP efflux from human trophoblast cell lines: a role for Multidrug Resistance Protein (MRP)1 transporter." in: Molecular human reproduction, 2010 (PubMed).

Product: Larsen, Bzorek, Pass et al.: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, 2011 (PubMed). Further details: incubated over night at room temperature, with primary antibody MEM- G1 in a concentration of 10 ?g/ml, diluted in wash buffer