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Details for Product No. ABIN94366

HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

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Antigen
Synonyms MHC-G, B-F, B-F-S04, B-F-S05, B-F-S06, B-F-S07, B-FI, B-FIV, BF2, BFa2, BFw-03, BFw-05, BFz-01
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 8 references available
Catalog no. ABIN94366
Quantity 0.1 mg
Price
348.48 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen Denatured bacterially expressed recombinant human HLA-G heavy chain.
Clone MEM-G-1
Isotype IgG1
Specificity The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to theMHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface oftrophoblast cells.
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Alternative Name HLAG
Research Area Major Histocompatibility Complex (MHC)
Application Notes Immunohistochemistry (paraffin sections) - Recommended dilution: 1: 60 to 1: 100Incubation: 1 h at RTPositive tissue: Human placenta - extravillous cytotrophoblastStaining technique: Standard ABC techniquePretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C
Product cited in: Larsen, Bzorek, Pass et al.: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, 2011 (PubMed). Further details: incubated over night at room temperature, with primary antibody MEM- G1 in a concentration of 10 ?g/ml, diluted in wash buffer

Fournel, Huc, Aguerre-Girr et al.: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).

Frumento, Franchello, Palmisano et al.: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, 2000 (PubMed).

Hurks, Valter, Wilson et al.: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, 2001 (PubMed).

Boyson, Erskine, Whitman et al.: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).

Shih, Kurman: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, 2004 (PubMed).

Gonen-Gross, Achdout, Arnon et al.: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Biondi, Ferretti, Lunghi et al.: "cAMP efflux from human trophoblast cell lines: a role for Multidrug Resistance Protein (MRP)1 transporter." in: Molecular human reproduction, 2010 (PubMed).

Alternatives for antigen "HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)", type "Antibodies"
Hosts (205), (69), (1)
Reactivities (270), (4), (1), (1)
Applications (174), (108), (98), (56), (56), (55), (38), (30), (18), (14), (12), (7), (2), (1), (1)
Conjugates (30), (29), (22), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Epitopes (15), (8), (5), (2), (2), (1), (1)
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