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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Heavy Chain) antibody

Details for Product No. ABIN94366, Supplier: Log in to see
Antigen
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
  • MHC-G
Epitope
Heavy Chain
32
17
8
7
7
7
6
6
6
5
4
2
2
2
1
1
1
1
1
Reactivity
Human
329
8
6
3
1
Host
Mouse
235
70
26
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
42
38
27
19
13
5
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
170
168
160
88
62
52
38
35
28
18
7
2
2
1
1
1
1
1
Supplier
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Immunogen Denatured bacterially expressed recombinant human HLA-G heavy chain.
Clone MEM-G-1
Isotype IgG1
Specificity The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name HLAG (HLAG Antibody Abstract)
Research Area Major Histocompatibility Complex (MHC)
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 1: 60 to 1: 00
Incubation: 1 h at RT
Positive tissue: Human placenta - extravillous cytotrophoblast
Staining technique: Standard ABC technique
Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Immunohistochemistry (IHC) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Heavy Chain) antibody (ABIN94366) Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary di...
Immunohistochemistry (IHC) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Heavy Chain) antibody (ABIN94366) Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary di...
Product cited in: Jasinski-Bergner, Stehle, Gonschorek, Kalich, Schulz, Huettelmaier, Braun, Seliger: "Identification of 14-3-3? Gene as a Novel miR-152 Target Using a Proteome-based Approach." in: The Journal of biological chemistry, Vol. 289, Issue 45, pp. 31121-35, 2014

Amiot, Vu, Rauch, LHelgoualch, Chalmel, Gascan, Turlin, Guyader, Samson: "Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis." in: Journal of hepatology, Vol. 60, Issue 2, pp. 245-52, 2014

Larsen, Bzorek, Pass, Larsen, Nielsen, Svendsen, Lindhard, Hviid: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, 2011 Further details: incubated over night at room temperature, with primary antibody MEM- G1 in a concentration of 10 ?g/ml, diluted in wash buffer

Biondi, Ferretti, Lunghi, Medici, Cervellati, Pavan, Vesce, Morano, Adinolfi, Bertoni, Abelli: "cAMP efflux from human trophoblast cell lines: a role for Multidrug Resistance Protein (MRP)1 transporter." in: Molecular human reproduction, 2010

Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005

Shih, Kurman: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, 2004

Boyson, Erskine, Whitman, Chiu, Lau, Koopman, Valter, Angelisova, Horejsi, Strominger: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002

Hurks, Valter, Wilson, Hilgert, van den Elsen, Jager: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, 2001

Frumento, Franchello, Palmisano, Nicotra, Giacomini, Loke, Geraghty, Maio, Manzo, Natali, Ferrara: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, 2000

Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000