HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (5A6G7)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN94380

Additional Information

Alternative name: HLA-G

Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.

Format: Purified

Isotype: IgG1

Clone: 5A6G7

Specificity: The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded byshedding from membrane-bound HLA-G forms that do not contain the intron 4-encodedepitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively splicedmRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and itis expressed on the surface of trophoblast cells.

Application Details

Application Notes: Western Blotting - Positive control: JEG-3 cell lysate. Application note: reducing conditions, 12% AA SDS-PAGE. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 - 20 µg/ml. Positive tissue: placenta tissue. Immunohistochemistry (frozen sections) - Recommended dilution: 10 - 20 µg/ml. Positive tissue: placenta tissue. ELISA - Positive control: HeLa/HLA-G5 transfectants cell lysateHeLa/HLA-G5 cell supernatant. Negative control: HeLa cell lysate. Application note: The antibody 5A6G7 has been tested as the capture antibody in asandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100).

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Western Blotting

Publications

Publications: Gonzalez, Alegre, Torres et al.: "Evaluation of HLA-G5 Plasmatic Levels During Pregnancy and Relationship with the 14-bp Polymorphism." in: American journal of reproductive immunology (New York, N.Y. : 1989), 2010 (PubMed).

Giuliani, Fleury, Vernochet et al.: "Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation." in: PLoS ONE, Vol. 6, Issue 5, pp. e19988, 2011 (PubMed).

Platonova, Cherfils-Vicini, Damotte et al.: "Profound coordinated alterations of intratumoral NK cell phenotype and function in lung carcinoma." in: Cancer research, Vol. 71, Issue 16, pp. 5412-22, 2011 (PubMed).

Favier, Howangyin, Wu et al.: "Tolerogenic Function of Dimeric Forms of HLA-G Recombinant Proteins: A Comparative Study In Vivo." in: PLoS ONE, Vol. 6, Issue 7, pp. e21011, 2011 (PubMed).