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Antibodies
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anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)
| Antigen |
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| Synonyms |
MHC-G |
| Clonality |
Monoclonal (5A6G7) |
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Host
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Reactivity
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Conjugate
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Application
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Alternatives Flow Cytometry (FACS), Western Blotting (WB), ELISA
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| Catalog no. |
ABIN94381 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
429.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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HLA-G
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Immunogen
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C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.
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Isotype
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IgG1
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Clone
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5A6G7
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Specificity
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The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded byshedding from membrane-bound HLA-G forms that do not contain the intron 4-encodedepitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively splicedmRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and itis expressed on the surface of trophoblast cells.
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Application Notes
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The reagent is intended for, ELISA, indirect Flow Cytometry analysis and immunocytochemistry. Suggested working dilution for is 1: 1000.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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1 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Major Histocompatibility Complex (MHC)
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Restrictions
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For Research Use only
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Alternatives for antigen "HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)", type "Antibodies"
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Hosts
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Mouse (74)
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Reactivities
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Human (74)
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Applications
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Flow Cytometry (FACS) (49), ELISA (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Western Blotting (WB) (19), Immunoprecipitation (IP) (17), Immunocytochemistry (ICC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Functional Studies (Func) (3), Immunohistochemistry (IHC) (2), Immunofluorescence (IF) (1)
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Conjugates
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FITC (11), Biotin (10), PE (10), Alexa Fluor 488 (7), APC (2), HRP (2)
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