V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)

Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN94783

Additional Information

Alternative name: AKT

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Format: Liquid (sterile filtered)

Description: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.

Specificity: This antiserum is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.

Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details

Application Notes: Recommended Dilutions: This product was assayed by immunoblot against a recommended positive control lysate. A single band of the expected apparent molecular weight was observed at a 1:500 dilution. For IHC (formalin-fixed paraffin-embedded) a 1:1,000 to 1:1,500 dilution is recommended. For immunofluorescence microscopy a 1:80 dilution is recommended on paraformaldehyde-fixed primary cardiomyocyte cultures. Researchers should determine optimal titers for other applications. Note: This antibody is suitable for western blotting, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture and ELISA.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.1% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage.Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product isstable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date one (1) year from opening.

Research Area: Signaling

Restrictions: For Research Use only

Images

Immunofluorescence microscopy. Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti- AKT staining appears green. Actin filaments are labeled red using a Texas-red

Immunoblotting. Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot. Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer. After washing, a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (ABIN102010) preceded color development using Pierce Chemical's SuperSignal