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CD8b Molecule (CD8B) antibody (Biotin)

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Antigen
  • Cd8b
  • Ly-3
  • Ly-C
  • Lyt-3
  • CD8B1
  • LEU2
  • LY3
  • LYT3
  • P37
  • Cd8b1
Reactivity
Mouse (Murine)
117
86
81
35
10
7
3
3
3
3
2
2
1
1
1
1
1
Host
Rat
152
98
58
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
52
42
25
11
5
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
214
111
80
53
28
26
13
13
11
10
4
3
2
2
2
1
1
1
1
Supplier
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Clone CT-CD8b
Isotype IgG2a
Specificity This anti-mouse CD8β Chain monoclonal antibody reacts with the mouse CD8 chain. The CD8 β Chain is also named Ly-3. The CD8 chain associates with the CD8 chain to form the CD8 / heterodimer on MHC class I restricted T-cells (cytotoxic T cells) and most thymocytes1. Since a small subset of T cells (IEL cells) express CD8 / homodimers rather than CD8 / heterodimers, investigators should use an antibody directed against the CD8 chain (CL168B, clone CT-CD8a) to definitively label mouse CD8 cells.
Alternative Name CD8 beta (CD8B Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain
Gene ID 12526
NCBI Accession NP_033988
UniProt P10300
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.1 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Should this product contain a precipitate we recommend microcentrifugation before use.
Background publications Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Tomonari, Spencer: "Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity." in: International immunology, Vol. 2, Issue 12, pp. 1189-94, 1991 (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).