Human Complement C5. Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of human serum or plasma. Cross-reactivity The antiserum does not cross-react with any other component of human plasma. Inter-species cross- reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
C5
Reactivity: Human
WB, IA
Host: Mouse
Monoclonal
568
unconjugated
Application Notes
Approved: IHC
Usage: The fluorescent immunoconjugate to human C5 is used to determine the presence and pattern using immunohistochemical staining techniques. Determinations of individual C components can be very useful in defining the exact location of a defect. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Sterile distilled water 1 ml
Concentration
Lot specific
Buffer
Lyophilized from PBS, pH 7.2
Handling Advice
Avoid freeze/thaw cycles. Store undiluted. Sensitive to light.
Storage
4 °C,-20 °C
Storage Comment
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted. Sensitive to light.