Akt (Ab-308) antibody detects endogenous levels of total Akt protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-TP-F-C).
AKT1
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Tremblay, Krebs, Dombrowski, Brehm, Bernroider, Roth, Nowotny, Waldhäusl, Marette, Roden: "Overactivation of S6 kinase 1 as a cause of human insulin resistance during increased amino acid availability." in: Diabetes, Vol. 54, Issue 9, pp. 2674-84, (2005) (PubMed).
Samuels, Diaz, Schmidt-Kittler, Cummins, Delong, Cheong, Rago, Huso, Lengauer, Kinzler, Vogelstein, Velculescu: "Mutant PIK3CA promotes cell growth and invasion of human cancer cells." in: Cancer cell, Vol. 7, Issue 6, pp. 561-73, (2005) (PubMed).