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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Synonyms
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ... show more
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN965526
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Akt
Immunogen This antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).
Specificity Akt (Ab-473) Antibody detects endogenous levels of total Akt protein.
Synonyms AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Baudhuin, Jiang, Zaslavsky et al.: "S1P3-mediated Akt activation and cross-talk with platelet-derived growth factor receptor (PDGFR)." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 2, pp. 341-3, 2004 (PubMed).

Feng, Tamaskovic, Yang et al.: "Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 35510-7, 2004 (PubMed).

Lungu, Jin, Yamawaki et al.: "Cyclosporin A inhibits flow-mediated activation of endothelial nitric-oxide synthase by altering cholesterol content in caveolae." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48794-800, 2004 (PubMed).

Ayala, Thompson, Yang et al.: "High levels of phosphorylated form of Akt-1 in prostate cancer and non-neoplastic prostate tissues are strong predictors of biochemical recurrence." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6572-8, 2004 (PubMed).

Alternatives
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Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts Rabbit (370), Mouse (95), Sheep (6), Goat (3), Chicken (1)
Reactivities Human (464), Mouse (Murine) (288), Rat (Rattus) (284), Chicken (114), Dog (Canine) (111), Cow (Bovine) (93), Sheep (Ovine) (88), Pig (Porcine) (83), Rabbit (37), Monkey (21), Chimpanzee (5), Horse (Equine) (5), Cat (Feline) (4), Chemical (3), Xenopus laevis (2), Arabidopsis thaliana (1), Frog (1)
Applications Western Blotting (WB) (347), ELISA (231), Immunofluorescence (IF) (151), Immunohistochemistry (IHC) (115), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (84), Flow Cytometry (FACS) (77), Immunoprecipitation (IP) (39), Immunocytochemistry (ICC) (20), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Dot Blot (Dot) (7), Fluorescence Microscopy (FM) (5), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (DB) (1)
Conjugates Biotin (12), Alexa Fluor 350 (9), Alexa Fluor 488 (9), Alexa Fluor 555 (9), Alexa Fluor 647 (9), PE,Cy5.5 (7), HRP (5), PE (5), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2), APC (1), Agarose Beads (1), Atto 594 (1), CF (1), Sepharose (1)
Epitopes pSer473 (39), pThr308 (28), C-Term (21), pTyr474 (19), pSer473,pTyr474 (18), N-Term (15), Internal Region (14), pThr450 (12), pSer129 (8), Ser473 (7), pThr308,Internal Region (7), pTyr326 (7), Thr308 (6), pSer124 (4), pThr34 (4), Ser124 (3), Ser129 (3), Thr450 (3), pSer246 (3), AA 460-480 (2), Ser246 (2), pSer473,Internal Region (2), AA 2-480 (1), AA 302-311,pThr308 (1), AA 460-480,C-Term, pSer473 (1), AA 464-477 (1), AA 74-89 (1), AA 80 (1), C-Term,Thr450 (1), N-Term,Gly37 (1), Phosphospecific (1), Tyr326 (1), pSer269 (1), pSer308 (1), pThr500 (1), pThr72 (1), pTyr204 (1), pTyr312,pTyr316,pTyr315 (1)

Variants
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Variants