Checkpoint Kinase 1 (CHEK1) (C-Term) antibody

Antigen: Checkpoint Kinase 1 (CHEK1)

Synonyms: CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965880

Additional Information

Alternative name: CHEK1

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human CHEK1(Serine/threonine-protein kinase Chk1)

Description: CHEK1(Serine/threonine-protein kinase Chk1) involved in cell cycle arrest when dna damage has occurred or when unligated dna is present. CHEK1 binds to and phosphorylates CDC25A, CDC25B and CDC25C. The phosphorylation of CDC25C creates a binding site for 14-3-3 protein which inhibits CDC25C. This prevents activation of the CDC2-Cyclin B complex and prevents mitotic entry. The CHEK1 phosphorylates CDC25C on serine216. Chk1 plays a primary role in the protection of cells from death induced by replication fork stress, whereas p21 mediates through its role in regulating entry into S phase. Chk1 deficiency resulted in a premature onset of mitosis because of abnormal activation of cyclin B-Cdc2 and led to the activation of caspases 3 and 9 triggered by cytoplasmic release of cytochrome c. It belongs to the Ser/Thr family of protein kinases and NIM1 subfamily.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Restrictions: For Research Use only

Publications

Product: Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Flaggs, Plug, Dunks et al.: "Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes." in: Current biology : CB, Vol. 7, Issue 12, pp. 977-86, 1998 (PubMed).

Rodriguez, Meuth: "Chk1 and p21 cooperate to prevent apoptosis during DNA replication fork stress." in: Molecular biology of the cell, Vol. 17, Issue 1, pp. 402-12, 2005 (PubMed).

Arlander, Felts, Wagner et al.: "Chaperoning checkpoint kinase 1 (Chk1), an Hsp90 client, with purified chaperones." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2989-98, 2006 (PubMed).

Petermann, Maya-Mendoza, Zachos et al.: "Chk1 requirement for high global rates of replication fork progression during normal vertebrate S phase." in: Molecular and cellular biology, Vol. 26, Issue 8, pp. 3319-26, 2006 (PubMed).