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Keratin 8 (KRT8) antibody

Details for Product No. ABIN965966, Supplier: Login to see
Antigen
  • ck8
  • cyk8
  • k2c8
  • card2
  • krt2-5
  • MGC69490
  • KRT8
  • DKFZp468F2127
  • CARD2
  • CK-8
  • CK8
  • CYK8
  • K2C8
  • K8
  • KO
  • KRT2-8
  • AA960620
  • AL022697
  • AU019895
  • Card2
  • EndoA
  • Krt-2.8
  • Krt2-8
  • CYKER
  • DreK8
  • cb186
  • krt2-8
  • sb:cb186
  • wu:fa20h05
  • wu:fa95h10
  • wu:fb96c06
  • zf-K8
  • zfk8
  • KERATIN8
Reactivity
Human
479
105
95
24
24
23
18
17
7
5
5
4
4
3
2
2
1
1
1
1
Host
Mouse
259
237
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
21
15
6
4
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
323
202
162
145
119
83
72
66
51
26
14
11
4
2
2
2
1
1
1
Supplier
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Immunogen Cytoskeleton preparation from HeLa Cells
Clone B391
Isotype IgG1
Purification Concentrated.
Alternative Name Cytokeratin 8 (52.2 kDa) (KRT8 Antibody Abstract)
Background This antibody reacts with Cytokeratin 8 (52 and 45 kD) human polypeptide. This cytokeratin coexists With Cytokeratin 18 and is found in all non-squamous epithelium but not in squamous epithelium. It Reacts with the adenocarcinomas of ovary, gastrointestinal tract, prostate, and thyroid.
Pathways
Application Notes Immunohistochemistry: (Paraffin/Frozen): 1:50 -1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III. The optimal conditions should be determined by the individual laboratory. The Auto/Zyme predigestion or high temperature antigenic unmasking technique is recommended with this antibody when using paraffinembedded tissue sections.
Comment

Cellular Localization: Cytoplasm.
Recommended Positive Control: Colon, Lung, or Breast Carcinomas

Restrictions For Research Use only
Format Liquid
Concentration 35 mg/ml
Buffer 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).