You are viewing an incomplete version of our website. Please click to reload the website as full version.

MSH2 antibody

Details for Product No. ABIN966594, Supplier: Log in to see
Antigen
Reactivity
Human
166
46
27
11
5
3
3
2
1
1
1
1
1
Host
114
50
3
Clonality
Monoclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
114
48
48
42
39
21
20
17
12
7
3
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Isotype IgG1
Specificity Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
Purification Antibodies are purified by protein G affinity chromatography.
Background MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
Gene ID 4436
Research Area Cancer, DNA/RNA
Application Notes Western Blot: Dilution 1: 200- 1: 1,000
IHC(P): Dilution 1: 200- 1: 1,000
IHC(F): Dilution 1: 200- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Storage -20 °C
Background publications Papadopoulos, Nicolaides, Wei, Ruben, Carter, Rosen, Haseltine, Fleischmann, Fraser, Adams: "Mutation of a mutL homolog in hereditary colon cancer." in: Science (New York, N.Y.), Vol. 263, Issue 5153, pp. 1625-9, 1994 (PubMed).

Palombo, Hughes, Jiricny, Truong, Hsuan: "Mismatch repair and cancer." in: Nature, Vol. 367, Issue 6462, pp. 417, 1994 (PubMed).