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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) antibody

Antigen

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2)
Synonyms FCC1, COCA1, HNPCC, LCFS2, HNPCC1, AI788990
Clonality Monoclonal
Host
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
1 reference available
Catalog no. ABIN966594
Quantity 0.1mg
Price 330.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name MSH2
Gene ID 4436
Isotype IgG1
Description MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
Specificity Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).

Application Details
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Application Notes Western Blot: Dilution 1: 200- 1: 1,000
IHC(P): Dilution 1: 200- 1: 1,000
IHC(F): Dilution 1: 200- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Purification Antibodies are purified by protein G affinity chromatography.
Storage Store at -20°C.
Research Area Cancer, DNA/RNA
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Papadopoulos, Nicolaides, Wei et al.: "Mutation of a mutL homolog in hereditary colon cancer." in: Science (New York, N.Y.), Vol. 263, Issue 5153, pp. 1625-9, 1994 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2)", type "Antibodies"
Hosts Rabbit (51), Mouse (24)
Reactivities Human (69), Rat (Rattus) (28), Mouse (Murine) (24), Cow (Bovine) (7), Pig (Porcine) (6), Rabbit (4), Chicken (2), Dog (Canine) (2), Monkey (2), Zebrafish (2), Cat (Feline) (1), Fruit Fly (Drosophila melanogaster) (1), Primate (1)
Applications Western Blotting (WB) (39), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunofluorescence (IF) (25), ELISA (20), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (15), Immunocytochemistry (ICC) (4), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes AA 550-600 (3), Internal Region (3), C-Term (2), Center (2), N-Term (2), AA 347-362 (1)