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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BCL2 Binding Component 3 (BBC3) (C-Term) antibody
|Synonyms||JFY1, PUMA, PUMA/JFY1, BBC3|
Human, Mouse (Murine), Rat (Rattus)
Alternatives Western Blotting (WB)
|4 references available|
|Price||316.25 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 5 to 7 Business Days|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequence.|
|Reconstitution||0.2ml of distilled water will yield a concentration of 500µg/ml.|
|Description||The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family.1, 2 The PUMA gene is located at 19q.3 PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation.4 Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.5|
|Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4|
|Preservative||0.05mg Thiomersal, 0.05mg NaN3.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month.|
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
|Restrictions||For Research Use only|
Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).
Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).
Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).
Chipuk, Bouchier-Hayes, Kuwana et al.: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." in: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, 2005 (PubMed).
|Hosts||Rabbit (63), Mouse (9), Goat (2)|
|Reactivities||Human (70), Mouse (Murine) (27), Rat (Rattus) (23), Cow (Bovine) (18), Dog (Canine) (18), Pig (Porcine) (18)|
|Applications||Western Blotting (WB) (59), ELISA (20), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (13), N-Term (8), AA 76-170 (4), Internal Region (2), AA 134-154 (1), AA 2-16 (1), BH3 Domain (1), Center (1)|