BCL2 Binding Component 3 (BBC3) (C-Term) antibody

Antigen: BCL2 Binding Component 3 (BBC3)

Synonyms: JFY1, PUMA, PUMA/JFY1, BBC3

Binding Site: C-Term

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN966924

Additional Information

Alternative name: PUMA

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequence.

Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Description: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family.1, 2 The PUMA gene is located at 19q.3 PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation.4 Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.5

Application Details

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative: 0.05mg Thiomersal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month.

Research Area: Cancer, Apoptosis/Necrosis, Metabolism

Restrictions: For Research Use only

Publications

Product: Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Chipuk, Bouchier-Hayes, Kuwana et al.: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." in: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, 2005 (PubMed).