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BBC3 antibody (BCL2 Binding Component 3) (C-Term)

Details for Product anti-BBC3 Antibody No. ABIN966924, Supplier: Log in to see
Antigen
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
Alternatives
anti-Human BBC3 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
88
43
21
13
13
11
8
7
6
3
3
2
2
1
1
1
1
Reactivity
Human
248
74
55
2
1
1
Host
Rabbit
220
25
13
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
10
9
7
7
7
5
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
235
71
55
44
35
30
11
9
6
4
3
2
2
Supplier
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Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.
Isotype IgG
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Bcl-2-binding component 3 (BBC3) detection. Tested with WB in Human, Mouse, Rat.
Alternative Name Bcl-2-binding component 3 (BBC3) (BBC3 Antibody Abstract)
Background The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
Pathways p53 Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Sodium azide, Thimerosal (Merthiolate)
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Product cited in: Chipuk, Bouchier-Hayes, Kuwana et al.: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." in: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, 2005 (PubMed).

Background publications Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).