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Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) antibody

Antigen

Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR)
Synonyms Flk1, Ly73, Flk-1, Krd-1, VEGFR2, VEGFR-2, sVEGFR-2, 6130401C07, FLK1, CD309, VEGFR, Vegfr-2, MGC93590, KDR, FLK-1, flk-1, vegfr-2
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN967248
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Alternative name VEGFR2
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1214 (F-H-YP-D-N).
Specificity VEGFR2 (Ab-1214) Antibody detects endogenous levels of total VEGFR2 protein.

Application Details
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Application Notes IHC: 1:50-1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Takahashi, Yamaguchi, Chida et al.: "A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-gamma and DNA synthesis in vascular endothelial cells." in: The EMBO journal, Vol. 20, Issue 11, pp. 2768-78, 2001 (PubMed).

Lamalice, Houle, Jourdan et al.: "Phosphorylation of tyrosine 1214 on VEGFR2 is required for VEGF-induced activation of Cdc42 upstream of SAPK2/p38." in: Oncogene, Vol. 23, Issue 2, pp. 434-45, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR)", type "Antibodies"
Hosts Rabbit (198), Mouse (33), Rat (16), Escherichia Coli (E. coli) (2), Goat (2), Chicken (1)
Reactivities Human (203), Mouse (Murine) (109), Rat (Rattus) (78), Dog (Canine) (20), Chicken (19), Cow (Bovine) (19), Pig (Porcine) (18), Cat (Feline) (1)
Applications Western Blotting (WB) (167), Immunohistochemistry (IHC) (88), ELISA (85), Immunofluorescence (IF) (71), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), Flow Cytometry (FACS) (57), Immunoprecipitation (IP) (17), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Neutralization (Neut) (5), Functional Studies (Func) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (14), PE (4), APC (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), FITC (2), PE,Cy5.5 (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), E-Tag (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr951 (20), C-Term (18), pTyr1175 (15), pTyr1214 (13), Internal Region (5), pTyr996 (4), N-Term (3), Tyr1175 (3), Tyr1214 (3), Tyr951 (3), pTyr1054 (3), pTyr1059 (3), AA 1158-1345 (2), pY1054 (2), AA 1057-1061 (1), AA 20-762 (1), AA 20-764 (1), Extracellular Domain (1), Extracellular,AA 20-764 (1), pTyr1054, pTyr1059 (1), pY951 (1)