Integrin beta 1 (ITGB1) antibody

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Antigen
  • cd29
  • fnrb
  • gpiia
  • itgb1
  • mdf2
  • msk12
  • vla-beta
  • vlab
  • CD29
  • 4633401G24Rik
  • AA409975
  • AA960159
  • ENSMUSG00000051907
  • Fnrb
  • Gm9863
  • gpIIa
  • FNRB
  • GPIIA
  • MDF2
  • MSK12
  • VLA-BETA
  • VLAB
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
  • integrin beta 1 (fibronectin receptor beta)
  • integrin, beta 1
  • itgb1-b
  • ITGB1
  • Itgb1
Alternatives
anti-Mouse (Murine) Integrin beta 1 antibody for Blocking Peptide
Reactivity
Mouse (Murine)
684
275
160
87
68
21
18
16
13
12
12
11
4
3
3
2
2
2
2
2
2
2
1
1
1
1
Host
Rat
502
197
73
50
38
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
72
69
47
29
28
24
21
18
17
16
16
12
12
12
12
12
11
11
11
8
8
7
4
4
4
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
569
412
214
172
139
138
93
89
55
13
13
13
10
10
9
7
7
6
4
3
3
3
2
2
1
1
1
1
1
Options
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Brand BD Pharmingen™
Immunogen (C57BL/6 x DBA/2)F1 mouse bone-marrow stromal cell clone BMS2
Clone KMI6
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD29 (ITGB1 Antibody Abstract)
Background The KMI6 antibody reacts with the 130-kDa integrin ß1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avß1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different ß1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.
Synonyms: Integrin beta1 chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex
Application Notes Please note that the epitope is lost upon sulfhydryl reduction of the antigen. For IHC, we recommend the use of purified anti-mouse CD29 mAb 9EG7 mAb in our special formulation for immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Product cited in: Wadsworth, Chang, Hong, Halvorson, Otto, Coligan: "Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 5, pp. 2125-33, 1995 (PubMed).

Wu, Miyake, Medina, Kincade, Gimble: "Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody." in: Hybridoma, Vol. 13, Issue 5, pp. 409-16, 1995 (PubMed).

Jacobsen, Miyake, Kincade, Osmond: "Highly restricted expression of a stromal cell determinant in mouse bone marrow in vivo." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 927-35, 1992 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).