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Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody

Details for Product No. ABIN967646
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Antigen
Synonyms
AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO, ZC2HC9, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKB ... show more
AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO, ZC2HC9, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, CG16910, DmIKK-gamma, DmIKKgamma, Dmel\\CG16910, Dmikkgamma, IKK, IKK[[gamma]], IKKg, IKKgamma, KEY, Kenny, Key, dIKK, dIKK-gamma, dmIKKgamma show less
Reactivity
Human
(214), (48), (46), (25), (14), (2), (2)
Host
Mouse
(181), (35), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(161), (105), (50), (39), (37), (23), (22), (20), (2), (1)
Pubmed 7 references available
Quantity 0.1 mg
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Catalog No. ABIN967646
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Immunogen Recombinant Human IKKgamma
Clone C73-764
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name IKK gamma
Background The transcription factor NF-kappaB, (nuclear factor kappa-B) is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is a critical step in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (aka IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKgamma migrates as a doublet between 50-55 kDa in SDS-PAGE.
Synonyms: NEMO
Molecular Weight 50-55 kDa doublet
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Application Notes Applications include western blot analysis and immunoprecipitation. In immunoprecipitation experiments, it is reported that the C73-764 antibody brings down the IKK complex (IKKalpha, IKKbeta, and IKKgamma). The immunoprecipitation application has not been evaluated. NIH/3T3 cells (ATCC CRL 1658) are recommended as a western blot positive control.
Comment

Related Products: ABIN968538, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody Western blot analysis of IKKgamma. Lysates from NIH/3T3 mouse embryonic fibroblast cells were probed with anti-IKKgamma antibody (clone C73-764) at a concentration of 0.5 myg/ml. IKKgamma is identified as a protein of 50-55 kDa (doublet).
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (2) anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (Image 2)
Product cited in: DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Nakano, Shindo, Sakon et al.: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998 (PubMed).

Rothwarf, Zandi, Natoli et al.: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998 (PubMed).

Li, Chu, Hu et al.: "The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1839-45, 1999 (PubMed).

Chu, Ostertag, Li et al.: "JNK2 and IKKbeta are required for activating the innate response to viral infection." in: Immunity, Vol. 11, Issue 6, pp. 721-31, 2000 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967646
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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