GAD65 antibody

Catalog No. ABIN967657

Product Details anti-GAD65 Antibody

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human, Rat, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified Rat GAD65

Clone: GAD-6

Isotype: IgG2a

Application Details

Application Notes: Rat brain cortex is recommended as a positive control for western blot

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-GAD65 antibody (ABIN967657)

Kanaani, Lissin, Kash, Baekkeskov: "The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65." in: The Journal of biological chemistry, Vol. 274, Issue 52, pp. 37200-9, (2000) (PubMed).

Hsu, Thomas, Chen, Davis, Foos, Chen, Wu, Floor, Schloss, Wu: "Role of synaptic vesicle proton gradient and protein phosphorylation on ATP-mediated activation of membrane-associated brain glutamate decarboxylase." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24366-71, (1999) (PubMed).

Esclapez, Tillakaratne, Kaufman, Tobin, Houser: "Comparative localization of two forms of glutamic acid decarboxylase and their mRNAs in rat brain supports the concept of functional differences between the forms." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 3 Pt 2, pp. 1834-55, (1994) (PubMed).

Kim, Richter, Aanstoot, Shi, Fu, Rajotte, Warnock, Baekkeskov: "Differential expression of GAD65 and GAD67 in human, rat, and mouse pancreatic islets." in: Diabetes, Vol. 42, Issue 12, pp. 1799-808, (1993) (PubMed).

Bu, Erlander, Hitz, Tillakaratne, Kaufman, Wagner-McPherson, Evans, Tobin: "Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 6, pp. 2115-9, (1992) (PubMed).

Chang, Gottlieb: "Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 6, pp. 2123-30, (1988) (PubMed).

Kaufman, McGinnis, Krieger, Tobin: "Brain glutamate decarboxylase cloned in lambda gt-11: fusion protein produces gamma-aminobutyric acid." in: Science (New York, N.Y.), Vol. 232, Issue 4754, pp. 1138-40, (1986) (PubMed).

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD65 (GAD2 Products)

Synonyms: GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody

Background: Gamma-aminobutyric acid (GABA) is an amino acid neurotransmitter that is considered to be the major inhibitory neurotransmitter in the mammalian central nervous system. The highest concentrations of GABA are found in the brain, where it is synthesized from glutamic acid to GABA by an enzyme called glutamic acid decarboxylase (GAD). GAD is also expressed in the insulin-producing beta cells of the islets of Langerhans. Two isoforms of GAD are present in rat brain, GAD65 and GAD67, based on their relative molecular weight in kDa. Both isoforms have significant levels of homology in the catalytic portion of the molecule, but differ greatly in the first 95 amino acids in the N-terminal region. GAD65 migrates at ~65 kDa in SDS/PAGE. The antibody is reported to recognize rat, human, mouse, and pig GAD65. Purified GAD65 from rat brain was used as the immunogen. The specific epitope recognized by this clone is a linear epitope localized in the last 41 amino acids of GAD65.

Molecular Weight: 65 kDa