Images for product: anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody
Western Blot analysis of Sox2 in Human Neural Stem Cells (NSC) and Mouse Embryonic Stem (ES) cell line. Lysates of NSC derived from H9 human ES cells (WiCell, Madison, WI, Left) and ES-E14TG2a mouse ES cells (ATCC CRL-1821, right) were probed with Purified Mouse anti-Sox2 monoclonal antibody at concentrations of 0.125 (lanes 1), 0.06 (lanes 2), and 0.03 myg/ml (lanes 3). Human and mouse Sox2 were identified as bands of ~36 kDa.
Immunoflourescent staining of Sox2 in human embryonic stem (ES) cells. H9 human ES cells (WiCell Madison, WI), passage 52 grown in mTESR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix, were fixed with BD Cytofix™ Fixation Buffer, permeabilized with BD™ Phosflow Perm Buffer III (Cat No. 558050), and stained with Purified Mouse anti-Sox2 monoclonal antibody (pseudo colored green) at 10 mg/mL. The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Permeabilization with BD Perm/Wash™ (Cat No. 554723) or 0.1% Triton™ X-100 buffer is also suitable for use with this antibody.