Janus Kinase 1 (JAK1) (AA 551-766) antibody

Antigen: Janus Kinase 1 (JAK1)

Synonyms: JTK3, JAK1A, JAK1B, AA960307, MGC37919, C130039L05Rik, MAPK8, JNK, JNK1, JAK1, jak1

Binding Site: AA 551-766

Clonality: Monoclonal (73)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN967842

Additional Information

Alternative name: JAK1

Immunogen: Human JAK1

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken, Frog

Format: Liquid

Isotype: IgG2b

Clone: 73

Description: The JAK family of receptor-associated protein kinases is directly involved in interferon (IFN) response pathways. The JAK family contains at least three members: JAK1, JAK2, and Tyk2. Each protein is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among family members. In several human and murine cell lines, JAK1 is rapidly tyrosine phosphorylated in response to IFN-alpha and IFN-gamma. JAK1 is required for the phosphorylation of the transcription factor Stat1 (p91), in response to IFNs-alpha or IFN-gamma. JAK1 is also necessary for the efficient phophorylation of Stat2 (p113) in response to IFN-alpha and for the phosphorylation of Tyk2 or JAK2 in response to IFNs-alpha or IFN-gamma, respectively.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Molecular Weight: 130 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Cytokines, Receptors, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of JAK1 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-JAK1.

Immunofluorescent staining of Human Endothelial cells.

Publications

Product: Nicholson, Oates, Harpur et al.: "Tyrosine kinase JAK1 is associated with the granulocyte-colony-stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 8, pp. 2985-8, 1994 (PubMed).

Müller, Briscoe, Laxton et al.: "The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction." in: Nature, Vol. 366, Issue 6451, pp. 129-35, 1993 (PubMed).

Kawazoe, Naka, Fujimoto et al.: "Signal transducer and activator of transcription (STAT)-induced STAT inhibitor 1 (SSI-1)/suppressor of cytokine signaling 1 (SOCS1) inhibits insulin signal transduction pathway through modulating insulin receptor substrate 1 (IRS-1) phosphorylation." in: The Journal of experimental medicine, Vol. 193, Issue 2, pp. 263-9, 2001 (PubMed).

Blesofsky, Mowen, Arduini et al.: "Regulation of STAT protein synthesis by c-Cbl." in: Oncogene, Vol. 20, Issue 50, pp. 7326-33, 2001 (PubMed).

Kopantzev, Heller, Swaminathan et al.: "IL-6 mediated activation of STAT3 bypasses Janus kinases in terminally differentiated B lineage cells." in: Oncogene, Vol. 21, Issue 44, pp. 6791-800, 2002 (PubMed).