JAK1 antibody (pTyr1022)

Catalog No. ABIN7205612

This Rabbit Polyclonal antibody specifically detects JAK1 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for JAK1 antibody (pTyr1022) (ABIN7205612)

Target: JAK1 (Janus Kinase 1 (JAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-JAK1 Antibody

Binding Specificity: pTyr1022

Purpose: Rabbit Anti-JAK1 (phospho Tyr1022) Polyclonal Antibody

Specificity: Phospho-JAK1 (Y1022) Polyclonal Antibody detects endogenous levels of JAK1 protein only when phosphorylated at Y1022.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human JAK1 Phospho-Tyr1022

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:200-1:1000,IHC 1:100-1:300,ELISA 1:10000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for JAK1

Target: JAK1 (Janus Kinase 1 (JAK1))

Alternative Name: JAK1

Background: JAK1, JAK1A, JAK1B, Tyrosine-protein kinase JAK1, Janus kinase 1, JAK-1JAK1 encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase (Janus kinase 1) phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants.

Molecular Weight: 132kD

Gene ID: 3716

UniProt: P23458

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response