JUP antibody (AA 553-738)

Catalog No. ABIN967860

Product Details anti-JUP Antibody

Target: JUP (Junction Plakoglobin (JUP))

Binding Specificity: AA 553-738

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human gamma-Catenin aa. 553-738

Clone: 15-gamma

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-JUP antibody (ABIN967860)

Akins, Greer: "Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion." in: The Journal of comparative neurology, Vol. 499, Issue 6, pp. 979-89, (2007) (PubMed).

Mary, Charrasse, Meriane, Comunale, Travo, Blangy, Gauthier-Rouvière: "Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 285-301, (2002) (PubMed).

Merritt, Berika, Zhai, Kirk, Ji, Hardman, Garrod: "Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation." in: Molecular and cellular biology, Vol. 22, Issue 16, pp. 5846-58, (2002) (PubMed).

Peng, Mandai, Nakanishi, Ikeda, Asada, Momose, Shibamoto, Yanagihara, Shiozaki, Monden, Takeichi, Takai: "Restoration of E-cadherin-based cell-cell adhesion by overexpression of nectin in HSC-39 cells, a human signet ring cell gastric cancer cell line." in: Oncogene, Vol. 21, Issue 26, pp. 4108-19, (2002) (PubMed).

Müller, Choidas, Reichmann, Ullrich: "Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10173-83, (1999) (PubMed).

McCrea, Turck, Gumbiner: "A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin." in: Science (New York, N.Y.), Vol. 254, Issue 5036, pp. 1359-61, (1992) (PubMed).

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: gamma-Catenin (JUP Products)

Synonyms: ARVD12 antibody, CTNNG antibody, DP3 antibody, DPIII antibody, PDGB antibody, PKGB antibody, arvd12 antibody, ctnng antibody, dp3 antibody, dpiii antibody, pdgb antibody, pkgb antibody, plak antibody, D930025P04Rik antibody, PG antibody, cb80 antibody, danPG antibody, fb11b06 antibody, jup antibody, wu:fb11b06 antibody, junction plakoglobin antibody, junction plakoglobin L homeolog antibody, Junction plakoglobin antibody, junction plakoglobin a antibody, junction plakoglobin S homeolog antibody, JUP antibody, Jup antibody, jup antibody, jup.L antibody, Tsp_06656 antibody, plak antibody, jupa antibody, jup.S antibody

Background: Gamma-Catenin (plakoglobin) was identified as a compenent of desmosomes where it associates with desmoglein. gamma-Catenin and beta-Catenin are closely related proteins that have significant homology with the Drosophila armadillo protein. In addition to complexing with E-Cadherin, gamma-Catenin and beta-Catenin have been observed in association with the intracellular domain of N-Cadherin. It has been proposed that one molecule of alpha-Catenin and at least one molecule of beta-Catenin and gamma-Catenin simultaneously bind to a single cadherin molecule. A 19 amino acid sequence of desmoglein (Dsg1) was found to be critical for binding of gamma-Catenin. This region has significant homology to the catenin-binding domain of classical cadherins, thus suggesting a common mechanism for gamma-Catenin localization at both adherens junctions and desmosomes.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Molecular Weight: 82 kDa

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location