Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 385-650) antibody

Details for Product No. ABIN967984
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms C9orf12, RGD1311271, rIpk1, RIP, RIP1, D330015H01Rik, Rinp, Rip1, 2400006N03Rik, RP23-83I13.5, Rip
Epitope
AA 385-650
(15), (7), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(70), (37), (37), (24), (23)
Host
Mouse
(58), (11), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(50), (22), (20), (19), (14), (7), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 50 µg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967984
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human RIP
Clone EB2
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name RIP
Background Binding or cross linking of the Fas antigen (also known as APO-1 and CD95) is known to elicit apoptosis in susceptible cells. Fas is a member of a family of cell surface receptors which includes tumor necrosis factor receptors (TNF-R, and TNF-R2) and nerve growth factor receptors (NGF-R), CD40, OX40, CD30, CD27, and 4-1BB. Several members of this family have been shown to regulate or induce cell death (TNF-R1 and TNF-R2). A 74 kDa member of this family protein named RIP (Receptor Interacting Protein) contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic death domain present in both Fas and TNF-R1. Both Fas and RIP have been shown to require this death domain to induce apoptosis and overexpression of RIP has been shown to induce cell death in transfected cells.
Synonyms: Receptor Interacting Protein
Molecular Weight 74 kDa
Research Area Cancer, Apoptosis/Necrosis
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 385-650) antibody Western blot analysis of RIP on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-RIP antibody.
anti-Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 385-650) antibody (2) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).
Product cited in: Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).

Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).

Lewis, Devin, Miller et al.: "Disruption of hsp90 function results in degradation of the death domain kinase, receptor-interacting protein (RIP), and blockage of tumor necrosis factor-induced nuclear factor-kappaB activation." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10519-26, 2000 (PubMed).

Fulda, Meyer, Debatin: "Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression." in: Cancer research, Vol. 60, Issue 14, pp. 3947-56, 2000 (PubMed).

Devin, Lin, Yamaoka et al.: "The alpha and beta subunits of IkappaB kinase (IKK) mediate TRAF2-dependent IKK recruitment to tumor necrosis factor (TNF) receptor 1 in response to TNF." in: Molecular and cellular biology, Vol. 21, Issue 12, pp. 3986-94, 2001 (PubMed).

Validation Images
Did you look for something else?
back to top