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Details for Product No. ABIN968218

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

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Antigen
Synonyms
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt,  ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF)
Pubmed 5 references available
Catalog no. ABIN968218
Quantity 150 µg
Price
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Immunogen Human Akt1 Recombinant Protein
Clone 55
Isotype IgG1, kappa
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Akt
Background Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases] is a family of serine/threonine kinases that contains a pleckstrin homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)], they are thought to be regulated similarly. Akt is activated by insulin and growth factors by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinase products bind to the PH domain, resulting in translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473. Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (Bad). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. The 55/PKBa/Akt monoclonal antibody recognizes Akt1, regardless of phosphorylation status. This antibody is routinely tested by western blot analysis.
Synonyms: PKBalpha, RAC-PK
Molecular Weight 59 kDa
Comment

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Datta, Dudek, Tao et al.: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." in: Cell, Vol. 91, Issue 2, pp. 231-41, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Ferrigno, Silver: "Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival." in: Oncogene, Vol. 18, Issue 45, pp. 6129-34, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts (655), (148), (9), (5), (4), (3)
Reactivities (794), (524), (502), (176), (154), (117), (95), (21), (19), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (591), (335), (205), (174), (143), (130), (117), (70), (31), (17), (17), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Conjugates (21), (15), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Epitopes (125), (77), (45), (44), (43), (35), (30), (28), (27), (18), (16), (16), (9), (9), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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