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RAD23 Homolog B (S. Cerevisiae) (RAD23B) (AA 73-193) antibody

Details for Product No. ABIN968319, Supplier: Log in to see
Antigen
  • 0610007D13Rik
  • AV001138
  • HHR23B
  • HR23B
  • MGC107846
  • mHR23B
  • P58
  • p58
  • zgc:65951
Alternatives
anti-Human RAD23 Homolog B (S. Cerevisiae) antibody for Western Blotting
Epitope
AA 73-193
26
16
16
13
9
8
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
114
36
33
3
3
3
2
1
1
1
1
Host
Mouse
70
35
9
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
92
37
18
18
16
11
6
4
3
3
1
1
1
Supplier
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Immunogen Human hHR23B aa. 73-193
Clone 16-hHR23B
Isotype IgG1
Cross-Reactivity Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name hHR23B (RAD23B Antibody Abstract)
Background Nucleotide excision repair (NER) is an essential element of DNA repair which eliminates unrelated DNA base lesions, including those induced by UV irradiation or chemicals. Although well defined in prokaryotes, eukaryotic NER is complex and involves at least two distinct subpathways. The transcription-coupled repair subpathway eliminates DNA lesions just prior to transcription. This is important for the removal of lesions for which the second pathway, global genome repair (i.e. covers the entire genome), is unable to repair. Individuals with defects in both pathways may suffer from xeroderma pigmentosum (XP), a condition with increased incidence of cancer in skin and internal organs. Mutations in seven genes result in human XP. However, one of these, XP-C, affects only the global genome repair pathway. The XP-C protein interacts with hHR23B, the human homolog of RAD23, an essential component of yeast NER. The XPC-hHR23B complex is required for excision of thymine dimers. In addition, hHR23B contains an N-terminal ubiquitin-like domain that allows for proteasome interaction. Thus, hHR23B may be an essential component of NER and mechanisms of DNA repair. This antibody is routinely tested by western blot analysis.
Molecular Weight 58 kDa
Pathways DNA Damage Repair
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-RAD23 Homolog B (S. Cerevisiae) (RAD23B) (AA 73-193) antibody (ABIN968319) Western blot analysis of hHR23B on a A431 cell lysate (Human epithelial carcinoma, AT...
Immunofluorescence (IF) image for anti-RAD23 Homolog B (S. Cerevisiae) (RAD23B) (AA 73-193) antibody (ABIN968319) Immunofluorescence staining of WiDr cells (Human colorectal adenocarcinoma, ATCC CCL-...
Product cited in: Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002

Wakasugi, Sancar: "Assembly, subunit composition, and footprint of human DNA repair excision nuclease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 12, pp. 6669-74, 1998

Reardon, Mu, Sancar: "Overproduction, purification, and characterization of the XPC subunit of the human DNA repair excision nuclease." in: The Journal of biological chemistry, Vol. 271, Issue 32, pp. 19451-6, 1996

Sugasawa, Masutani, Uchida, Maekawa, van der Spek, Bootsma, Hoeijmakers, Hanaoka: "HHR23B, a human Rad23 homolog, stimulates XPC protein in nucleotide excision repair in vitro." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4852-61, 1996

Masutani, Sugasawa, Yanagisawa, Sonoyama, Ui, Enomoto, Takio, Tanaka, van der Spek, Bootsma: "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23." in: The EMBO journal, Vol. 13, Issue 8, pp. 1831-43, 1994