SLC3A2 antibody (AA 9-204)

Catalog No. ABIN968570

Product Details anti-SLC3A2 Antibody

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Binding Specificity: AA 9-204

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC3A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat 4F2 hc aa. 9-204

Clone: 30-CD98HC

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968548

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SLC3A2 antibody (ABIN968570)

Nakamura, Sato, Yang, Miyagawa, Harasaki, Tomita, Matsuoka, Noma, Iwai, Minato: "4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 3009-16, (1999) (PubMed).

Tsurudome, Ito, Takebayashi, Okumura, Nishio, Kawano, Kusagawa, Komada, Ito: "Cutting edge: primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2462-6, (1999) (PubMed).

Kanai, Segawa, Miyamoto, Uchino, Takeda, Endou: "Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23629-32, (1998) (PubMed).

Fenczik, Sethi, Ramos, Hughes, Ginsberg: "Complementation of dominant suppression implicates CD98 in integrin activation." in: Nature, Vol. 390, Issue 6655, pp. 81-5, (1997) (PubMed).

Target Details for SLC3A2

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Alternative Name: CD98HC (SLC3A2 Products)

Synonyms: 4F2 antibody, 4F2HC antibody, 4T2HC antibody, CD98 antibody, CD98HC antibody, MDU1 antibody, NACAE antibody, Mdu1 antibody, MGC53951 antibody, slc3a2 antibody, MGC53965 antibody, SLC3A2 antibody, AI314110 antibody, Cd98 antibody, Ly-10 antibody, Ly-m10 antibody, Ly10 antibody, Mgp-2hc antibody, fc65h12 antibody, wu:fc65h12 antibody, id:ibd1327 antibody, sb:cb519 antibody, wu:fe14a07 antibody, wu:fq18d12 antibody, zgc:55813 antibody, solute carrier family 3 member 2 antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog antibody, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2a antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2b antibody, SLC3A2 antibody, Slc3a2 antibody, slc3a2.L antibody, slc3a2.S antibody, slc3a2a antibody, slc3a2b antibody

Target Type: Chemical

Background: 4F2 antigen (CD98) was identified as a heterodimer consisting of an 80 kDa type II glycosylated integral membrane protein 4F2 heavy chain (4F2 hc) linked by disulfide bonds to a hydrophobic, non-glycosylated, 37 kDa protein 4F2 light chain (4F2 lc). The 4F2 hc colocalizes with cadherins at cell adhesion sites and is required for the intracellular targeting of 4F2 heterodimers. 4F2 hc also associates with beta1 integrins and overexpression of 4F2 reverses the suppression of beta1 integrin activation caused by overexpression of beta1 cytoplasmic domains. 4F2 has also been identified as a fusion regulatory protein FRP-1, since anti-FRP-1 antibodies can induce HIV-mediated cell fusion via an integrin system. 4F2 heterodimers are also important for activation of amino acid transport. 4F2 hc is 30% homologous with the amino acid transport activator D2/rBAT and expression of 4F2 hc in Xenopus oocytes induces system y+L amino acid transport. Thus, 4F2 hc may participate in intracellular trafficking and activation of amino acid transporters, as well as in the regulation of integrin signaling.
Synonyms: 4F2 hc

Molecular Weight: 80 kDa