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CDK1 antibody (Cyclin-Dependent Kinase 1)

Details for Product anti-CDK1 Antibody No. ABIN969034, Supplier: Log in to see
Antigen
  • 5363
  • cdc
  • cdc-2
  • cdc2
  • Cdc2
  • CDC2
  • cdc2-a
  • Cdc2a
  • CDC2A
  • CDC2AAT
  • cdc2Dm
  • cdc2x1.1
  • cdc28a
  • CDC28A
  • CDCDm
  • Cdk-1
  • Cdk1
  • CDK1
  • cdk1
  • CDK1/CDC2
  • CDK2
  • CDKA1
  • CDKA;1
  • cell division control 2
  • CG5363
  • CYCLIN-DEPENDENT KINASE A;1
  • Dcdc2
  • dCdk1
  • Dmcdc2
  • Dm cdc2
  • DmCdc2
  • DmCdk1
  • Dmel\\CG5363
  • group 4
  • l(2)31Eh
  • p34
  • p34cdc2
  • P34CDC2
  • POL3
  • pold
  • PSTAIR
  • wu:fc30e01
  • xcdc2
  • zgc:92032
Alternatives
anti-Human CDK1 antibody for Immunocytochemistry
Reactivity
Human
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267
44
38
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31
28
22
21
10
9
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6
4
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
510
211
Clonality (Clone)
Monoclonal ()
Conjugate
This CDK1 antibody is un-conjugated
29
26
24
19
16
16
9
7
7
5
5
5
5
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5
2
2
2
2
2
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2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
584
278
173
129
110
79
60
54
50
36
10
10
7
6
6
2
1
1
1
1
Supplier
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Immunogen Purified recombinant fragment of CDC2 expressed in E. coli.
Clone 8C5A7F10
Isotype IgG1
Purification Ascites
Alternative Name CDC2 (CDK1 Antibody Abstract)
Background The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
Molecular Weight 34 kDa
Gene ID 983
HGNC 983
Research Area Cell Cycle
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-CDK1 antibody (Cyclin-Dependent Kinase 1) (ABIN969034) Figure 1: Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-CDK1 antibody (Cyclin-Dependent Kinase 1) (ABIN969034) Figure 2: Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green) . Re...
Western Blotting (WB) image for anti-CDK1 antibody (Cyclin-Dependent Kinase 1) (ABIN969034) Figure 3: Western blot analysis using CDC2 mouse mAb against Jurkat (1) cell lysate.
Product cited in: Meyer, Merkel, Brückl, Schellerer, Schildberg, Campean, Hohenberger, Croner: "Cdc2 as prognostic marker in stage UICC II colon carcinomas." in: European journal of cancer (Oxford, England : 1990), Vol. 45, Issue 8, pp. 1466-73, 2009 (PubMed).

Tang, Wu, Guo, Hansen, Perry, Freel, Nutt, Jackson, Kornbluth: "Cdc2 and Mos regulate Emi2 stability to promote the meiosis I-meiosis II transition." in: Molecular biology of the cell, Vol. 19, Issue 8, pp. 3536-43, 2008 (PubMed).