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MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5)

Details for Product anti-MAP3K5 Antibody No. ABIN969273, Supplier: Log in to see
Antigen
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
Alternatives
anti-Human MAP3K5 antibody for Western Blotting
Reactivity
Human
360
139
79
4
1
Host
Mouse
347
21
Clonality (Clone)
Monoclonal ()
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
6
5
5
5
5
5
5
5
5
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
279
140
124
56
50
37
19
11
6
6
5
2
1
1
1
Supplier
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Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. coli.
Clone 2-00E-04
Isotype IgG1
Purification Ascites
Alternative Name MAP3K5 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Molecular Weight 55 kDa
Gene ID 4217
HGNC 4217
Pathways MAPK Signaling
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 2: Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 3: Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green) . ...
Immunohistochemistry (IHC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 5: Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) ...
Product cited in: Widenmaier, Ao, Kim et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent ..." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009 (PubMed).